| EFFECT OF 764－3 ON AGGREGATION AND CALCIUM MOVEMENTS IN AEQUORIN－LOADED HUMAN PLATELETS |
| |
| 引用本文： | 武怀珠,李家增,彭林,滕彬,翟治清.EFFECT OF 764－3 ON AGGREGATION AND CALCIUM MOVEMENTS IN AEQUORIN－LOADED HUMAN PLATELETS[J].中国医学科学杂志,1996(1). |
| |
| 作者姓名： | 武怀珠 李家增 彭林 滕彬 翟治清 |
| |
| 摘 要： | ＥＦＦＥＣＴＯＦ７６４－３ＯＮＡＧＧＲＥＧＡＴＩＯＮＡＮＤＣＡＬＣＩＵＭＭＯＶＥＭＥＮＴＳＩＮＡＥＱＵＯＲＩＮ－ＬＯＡＤＥＤＨＵＭＡＮＰＬＡＴＥＬＥＴＳＷｕＨｕａｉｚｈｕ武怀珠；ＬｉＪｉａｚｅｎｇ李家增；ＰｅｎｇＬｉｎ彭林；ＴｅｎｇＢｉｎ滕彬ａｎｄＺ...
|
| 关 键 词： | antiplatelet drugs platelet aggregation platelet [Ca￣(2 )] i |
EFFECT OF 764-3 ON AGGREGATION AND CALCIUM MOVEMENTS IN AEQUORIN-LOADED HUMAN PLATELETS |
| |
| Wu Huaizhu, Li Jiazeng, Peng Lin, Teng Bin and Zhai Zhiqing.EFFECT OF 764-3 ON AGGREGATION AND CALCIUM MOVEMENTS IN AEQUORIN-LOADED HUMAN PLATELETS[J].Chinese Medical Sciences Journal,1996(1). |
| |
| Authors: | Wu Huaizhu Li Jiazeng Peng Lin Teng Bin and Zhai Zhiqing |
| |
| Abstract: | Washed human platelets were loaded with the Ca2 -sensitive photoprotein, aequorin. using hypoosmotic shock treatment-technique. Then aggregation and cytoplasmic ionized calcium concentration ( Ca2 ] i) changes in response to collagen or thrombin were measured simultaneously in the aequorin-loaded human platelets with a Platelet Ionized Calcium Aggregometer. 764-3. an active component isolated from the Chinese medicinal herb Salvia Miltiorrhiza Bge, inhibited platelet Ca2 ]i rise as well as aggregation evoked by collagen or thrombin in the presence of extracellular Ca2 . After the extracellular Ca2 . was removed by addition of EGTA, collagen or thrombin. causing no aggregation. still elicited platelet Ca2 ] i rise which reflected Ca2 mobilization from intraplatelet stores. Under this condition, 764-3 could also suppress platelet Ca2 ] i rise. Analysis shows that 764-3 inhibrts platelet Ca2 influx and Ca2 mobilization with similar potency. which accounts for its suppression of platelet Ca2 ] i rise, and must contribute to its inhibition of platelet aggregation. |
| |
| Keywords: | |
|
