| 自由基清除剂MCI-186对高糖环境中内皮细胞凋亡的干预作用 |
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| 引用本文： | 董叫云,高见佳宏,青春,田中秀治,山口亮,郭静苹,尾乡贤,陆树良.自由基清除剂MCI-186对高糖环境中内皮细胞凋亡的干预作用[J].中华糖尿病杂志,2005,13(3):234-235. |
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| 作者姓名： | 董叫云 高见佳宏 青春 田中秀治 山口亮 郭静苹 尾乡贤 陆树良 |
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| 作者单位： | [1]日本杏林大学医学院形成外科及热伤中心 [2]上海第二医科大学附属瑞金医院,上海市烧伤研究所,200025 |
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| 基金项目： | 国家重点研究发展规划项目资助(G1999054205) |
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| 摘 要： | 观察内皮细胞在高糖环境及经肿瘤坏死因子α(TNF-α)诱导后的细胞凋亡情况，并加入自由基清除剂MCI-186予以干扰。检测细胞DNA裂解片段及凋亡信号蛋白(caspase)的表达量，以期探讨MCI-186对高糖环境中血管内皮细胞凋亡的干预作用及凋亡机制。发现高糖环境可增加内皮细胞凋亡率，并上调TNF-α诱导的调亡。MCI-186可抑制高糖诱导的细胞凋亡。但无法干预高糖环境中TNF—a诱导的凋亡。
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| 关 键 词： | 自由基清除剂 MCI-186 高糖环境 内皮细胞 细胞凋亡 TNF-α 自由基 半胱氨酸天冬氨酸蛋白酶 |
| 修稿时间： | 2003年12月15 |
Effect of a radical scavenger (MCI 186) on glucose induced apoptosis of cultured human dermal microvascular endothelial cells(HDMEC) |
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| DONG Jiao-yun,Yoshihiro Takami,QING Chun,et al..Effect of a radical scavenger (MCI 186) on glucose induced apoptosis of cultured human dermal microvascular endothelial cells(HDMEC)[J].CHINESE JOURNAL OF DIABETES MELLITUS,2005,13(3):234-235. |
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| Authors: | DONG Jiao-yun Yoshihiro Takami QING Chun |
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| Institution: | DONG Jiao-yun*,Yoshihiro Takami,QING Chun,et al. *Burn Institute of Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China |
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| Abstract: | In the present study, the role of a free radical scavenger (MCI-186) in preventing endothelial cell apoptosis induced by high glucose condition was studied. HDMEC were incubated with high glucose medium, with or without MCI-186. After 7 days of incubation, cell apoptosis with or without TNF-alpha was detected through DNA fragment and caspase assay. High glucose condition could enhance cell apoptosis and up-regulate TNF-alpha induced cell apoptosis. Based on the data of caspase-8 and caspase-3, TNF-alpha induced EC apoptosis might occur through mitochondria death pathway dominantly on high glucose condition. MCI-186 might suppress high glucose-induced cell apoptosis, however, it did not alter TNF-alpha induced apoptosis. These results suggest that MCI-186 may be useful in preventing endothelial cell damages by high glucose condition. |
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| Keywords: | High glucose Endothelial cells Free radical Apoptosis Caspase |
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