Mixed vaginal infections of Balb/c mice with low virulent herpes simplex type 1 strains result in restoration of virulence properties: vaginitis/vulvitis and neuroinvasiveness |
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Authors: | M Lingen F Hengerer D Falke |
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Institution: | Institut für Virologie, J.-Gutenberg-Universit?t, Hochhaus am Augustusplatz, D-55101 Mainz, Germany Tel: 49-6131-17-7382; Fax: 49-6131-39-5604, DE
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Abstract: | Vaginal infections of BALB/c Ann mice with herpes simplex virus type 1 (HSV-1) were studied. Mice were inoculated with virulent
strains ANG path and 17 syn+ or low-virulent recombinant strains 27/III and 17-syn3 that differ from parental strains in their glycoprotein B (gB) gene
sequences. When low-virulent strains were inoculated separately, no vaginitis/vulvitis was produced despite replication in
the vagina. In contrast, after coinfection of mice with the two low-virulent strains, vaginitis/vulvitis was produced and
virus could be recovered from the central nervous system (CNS). Two of the CNS isolates produced vaginitis/vulvitis, neuroinvasiveness
and death of mice after vaginal infection. Restriction fragment analysis and sequencing were used to assess recombination
events in the gB gene sequence of the CNS isolates. After mixed vaginal infection recombination between non-virulent HSV strains
occurs, resulting in vaginitis/vulvitis and neuroinvasiveness. No correlation was detected between the syncytial phenotype
and local vaginal virulence. Virulence of HSV is not solely dependent on gB function; it seems to be more probable that several
genes act in concert to induce virulence and neuroivasiveness.
Received: 28 May 1996 |
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Keywords: | Herpes simplex virus Vaginal infection Neurovirulence Recombination |
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