Comparative analysis of the prion protein gene sequences in African lion |
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| Authors: | Chang-De Wu Wan-Yong Pang De-Ming Zhao |
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| Institution: | (1) National Animal Transmissible Spongiform Encephalopathies Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China;(2) College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Liaoning, 110161, China;(3) Faculty of Veterinary Medicine, University College Dublin, Dublin 4, Dublin, Ireland |
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| Abstract: | The prion protein gene of African lion (Panthera Leo) was first cloned and polymorphisms screened. The results suggest that the prion protein gene of eight African lions is highly
homogenous. The amino acid sequences of the prion protein (PrP) of all samples tested were identical. Four single nucleotide
polymorphisms (C42T, C81A, C420T, T600C) in the prion protein gene (Prnp) of African lion were found, but no amino acid substitutions.
Sequence analysis showed that the higher homology is observed to felis catus AF003087 (96.7%) and to sheep number M31313.1 (96.2%) Genbank accessed. With respect to all the mammalian prion protein sequences
compared, the African lion prion protein sequence has three amino acid substitutions. The homology might in turn affect the
potential intermolecular interactions critical for cross species transmission of prion disease |
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| Keywords: | Prion protein African lion Analysis |
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