双份脐血间CD34^＋细胞与CD3^＋细胞相互作用对分化增殖能力的影响

目的双份脐血移植的植入动力学机制目前尚无定论，推测双份脐血中的淋巴细胞与优势份脐血的产生相关。本实验将双份脐血的CD34^＋细胞与CD3^＋细胞混合培养，观察CD3^＋细胞对CD34^＋细胞的增殖分化有无影响。方法建立液体和半固体培养体系，将免疫磁珠分选纯化的双份脐血间的CD34^＋细胞和CD3^＋细胞混合培养6d和14d。以流式细胞计数观测CD34^＋细胞培养后的分化指标（CD33，CD41，CD71）；计数集落形成单位（GM—CFU、BFU-E、GEMM—CFU）分析CD34^＋细胞的增殖情况。结果液体共培养后各份CD34^＋细胞表面分化指标的变化。脐血CD34^＋细胞分选富集的纯度为（98．70±0．72）％。3d实验组和对照组的各项分化指标无差异（P〉0．05）；6d的CD33、CD71实验组明显低于对照组，而CD41明显高于对照组（P〈0．05）。半固体共培养后CD34^＋细胞增殖能力的变化。实验组的红系集落形成单位（BFU—E）及粒单细胞集落形成单位（GM—CFU）数低于对照组（P〈0．05），而混合细胞集落形成数（GEMM—CFU）高于对照组（P〈0．05）。结论将两份脐血的CD34^＋细胞和CD3^＋细胞体外混合培养对CD34^＋细胞的增殖分化能力有影响，推测双份脐血间的相互作用可部分地通过CD3^＋细胞介导。

Influence of ex vivo co-culture of CD34~+ cells and CD3~+ cells from different unit UCB on the differentiation and proliferation ability

Objective The early engraftment kinetics of the transplantation of double umbilical cord blood (CB/UCB) units are still not really understood. This study was aimed to explore the influence of ex vivo co-culture of CD34+ cells and CD3+ cells from different UCB units on the proliferation and differentiation ability.Methods CB CD34+ cells and CD3+ cells from different UCB units were co-cultured in the liquid and semisolid mediums. The differentiation abilities (CD33, CD41, CD71) were assessed respectively by f...