bFGF对培养兔关节软骨细胞作用的实验研究

目的：在于了解硷性成纤维细胞生长因子（ｂＦＧＦ）对关节软骨细胞分裂增殖及其特点以及功能代谢的影响。方法：体外单层培养兔关节软骨细胞，以１ｎｇ／ｍｌ、１０ｎｇ／ｍｌ、１００ｎｇ／ｍｌ的ｂＦＧＦ作用细胞２、４、６ｄ，测ＤＮＡ含量及基质中糖醛酸含量，阐明细胞的增殖及代谢的变化。同时，在１０ｎｇ／ｍｌｂＦＧＦ作用下，采用流式细胞技术进行细胞周期亚时相分析。结果：结果显示在１～１００ｎｇ／ｍｌ浓度范围内，ｂＦＧＦ对培养软骨细胞的增殖及代谢均有促进作用，以１０ｎｇ／ｍｌ浓度作用最佳。细胞周期较对照组明显缩短，ＤＮＡ合成前期（Ｇ１期），ＤＮＡ合成期（Ｓ期）和分裂前期及分裂期（Ｇ２Ｍ）的时间分别为１１５、１６３、７２ｈ，对照组分别为２２３、１７９、１５８ｈ。结论：ｂＦＧＦ对培养的关节软骨细胞增殖及基质代谢有促进作用，能缩短软骨细胞ＤＮＡ合成Ｇ１期及Ｇ２Ｍ期而达到缩短细胞周期、促进细胞分裂增殖的。

Effects of bFGF on Cultured Rabbit Articular Chondrocytes

Objective:To investigate the effects of basic fibroblast growth factor (bFGF) on the metabolism of articular chondrocytes and their matrix.Methods:The monolayer cells obtained from 1 month rabbit were cultured in DMEM containing 10% fetal calf serum and were treated with bFGF at doses ranging from 1 to 100 ng/ml over 2 day,4 day,6 day periods.Results:We found that bFGF stimulated in a doseand timedependent manner,cell proliferation and matrix metabolism,which were demonstrated by means of DNA content and GlcUA content.Maximal effects of stimulation occurred at 10ng/ml within 4day after treatment.We also investigated the effects of bFGF at 10 ng/ml on the cyclic change of the cell.The second generation of chondrocytes as well as control cells were cultured 72 hours,in terms of their cycles determined by flow cytometer.The results showed that the time of G1,DNA synthesis and G2M phases in control group were 223 hours,179 hours and 158 hours respectively while those G2M phase was shortened by bFGF. Flow cytometrywere 115 hours,163 hours and 72 hours respectirely in bFGF group.Conclusion:It was showed that the time needed for G1 phase and