| 多重耐药鲍曼不动杆菌16S rRNA甲基化酶、氨基糖苷类修饰酶基因研究 |
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| 引用本文： | 冯旰珠,张扬,姚堃,赵水娣.多重耐药鲍曼不动杆菌16S rRNA甲基化酶、氨基糖苷类修饰酶基因研究[J].中国抗感染化疗杂志,2008,8(4). |
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| 作者姓名： | 冯旰珠 张扬 姚堃 赵水娣 |
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| 作者单位： | 南京医科大学第二附属医院呼吸科 南京医科大学第二附属医院细菌室 |
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| 基金项目： | 南京医科大学重点研究基金 |
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| 摘 要： | 目的了解南京地区多重耐药鲍曼不动杆菌16S rRNA甲基化酶、氨基糖苷类修饰酶基因的存在状况。方法自2007年7—10月间南京地区住院病人标本中分离并筛选出20株多重耐药鲍曼不动杆菌,微量肉汤稀释法测定11种抗菌药物的敏感性;PCR法检测16S rRNA甲基化酶、氨基糖苷类修饰酶基因。结果20株多重耐药鲍曼不动杆菌16S rRNA甲基化酶(armA、rmtB)基因均为阴性;氨基糖苷类修饰酶aac(3)-Ⅰ基因阳性株10株、aac(3)-Ⅱ阳性株1株、aac(6′)-Ⅰb基因阳性株13株、ant(3″)-Ⅰ基因阳性株12株;ant(2″)-Ⅰ基因、aac(6′)-Ⅱ基因及aac(6′)-Ⅰad基因均为阴性。结论南京地区多重耐药鲍曼不动杆菌对氨基糖苷药物耐药的主要原因是aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3″)-Ⅰ4种氨基糖苷类修饰酶基因的存在;尚未检出氨基糖苷类修饰酶新亚型———aac(6′)-Ⅰad基因和16S rRNA甲基化酶armA、rmtB基因。
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| 关 键 词： | 鲍曼不动杆菌 甲基化酶 氨基糖苷类修饰酶 耐药性 多药 |
Study on the genes of 16S rRNA methylase and aminoglycoside modifying enzyme in multi-resistant Acinetobacter baumannii |
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| FENG Gan-zhu; ZHANG Yang; YAO Kun; ZHAO Shui-Di.Study on the genes of 16S rRNA methylase and aminoglycoside modifying enzyme in multi-resistant Acinetobacter baumannii[J].Chinese Journal of Infection and Chemotherapy,2008,8(4). |
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| Authors: | FENG Gan-zhu; ZHANG Yang; YAO Kun; ZHAO Shui-Di |
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| Institution: | FENG Gan-zhu; ZHANG Yang; YAO Kun; ZHAO Shui-Di.(Department of Respiratory Medicine; Second Affiliated Hospital of Nanjing Medical University; Nanjing 210011; China); |
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| Abstract: | Objective To survey the prevalence of genes encoding 16S rRNA methylase and aminoglycoside modifying enzyme in multi-resistant Acinetobacter baumannii.Methods The samples of 20 multi-resistant A.baumannii isolates were collected from inpatients from July 2007 to October 2007 in Nanjing.The sensitivity of the isolates to 11 antibacterial agents was determined using a broth induction method.The genes of 16S rRNA methylase and aminoglycoside modifying enzyme were analyzed by polymerase chain reaction.Results 1... |
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| Keywords: | Acinetobacter baumanii Methylase Aminoglycoside modifying enzyme Multidrug resistance |
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