| HPLC-蒸发光散射检测法测定贞芪扶正颗粒中黄芪甲苷的含量 |
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| 引用本文： | 董红红,张丽增,郭小青,秦雪梅,代云桃,张军.HPLC-蒸发光散射检测法测定贞芪扶正颗粒中黄芪甲苷的含量[J].中国药房,2008,19(6):444-446. |
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| 作者姓名： | 董红红 张丽增 郭小青 秦雪梅 代云桃 张军 |
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| 作者单位： | 山西大学化学化工学院药学系,太原市,030006 |
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| 摘 要： | 目的:建立高效液相色谱-蒸发光散射检测法测定贞芪扶正颗粒中黄芪甲苷含量的方法。方法:色谱柱为Hypersil ODS2 C18(200mm×4.6mm,5μm),流动相为乙腈-水(35∶65),流速为1mL·min-1,载气为氮气(N2),气体压力为1.5MPa,雾化温度为35℃,气化温度为50℃。结果:黄芪甲苷进样量在2.38~51.00μg范围内的对数值与峰面积对数值呈良好线性关系;平均回收率为100.71%,RSD=1.94%(n=6)。结论:本方法迅速、准确、重现性好,可用于贞芪扶正颗粒的质量控制。
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| 关 键 词： | 高效液相色谱-蒸发光散射检测法 贞芪扶正颗粒 黄芪甲苷 含量测定 |
| 文章编号： | 1001-0408（2008）06-0444-03 |
| 收稿时间： | 2007-03-12 |
| 修稿时间： | 2007-05-20 |
Determination of Astragoloside Ⅳ in Zhenqifuzheng Granule by HPLC-ELSD |
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| DONG Hong-hong,ZHANG Li-zeng,GUO Xiao-qing,QIN Xue-mei,DAI Yun-tao,ZHANG Jun.Determination of Astragoloside Ⅳ in Zhenqifuzheng Granule by HPLC-ELSD[J].China Pharmacy,2008,19(6):444-446. |
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| Authors: | DONG Hong-hong ZHANG Li-zeng GUO Xiao-qing QIN Xue-mei DAI Yun-tao ZHANG Jun |
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| Institution: | (Dept. of Pharmacy, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China) |
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| Abstract: | OBJECTIVE： To determine the content of Astragoloside IV in Zhenqifuzheng granule by HPLC- ELSD. METHODS： The samples were separated on Hypersil ODS2 C18（200 mm × 4.6 mm, 5 μm） with mobile phase consisted of acetonitrile - water （35 ： 65） at a flow rate of 1 mL · min^-1.The carrier gas using was N2 with a gas pressure of 0.15 MPa .The atomization temperature was 35 ℃ and the gasification temperature was 50 ℃ .RESULTS： This method showed a good linear relationship in the range of 2.38～51.00 μg for Astragaloside Ⅳ . The average recovery was 100.71% with RSD being 1.94% （ n = 6）. CONCLUSION ： The method is rapid, accurate and reproducible, and it can be used for the quality control of Zhenqi- fuzheng granule. |
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| Keywords: | HPLC - ELSD Zhenqifuzheng granule Astragoloside Ⅳ Content determination |
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