IL-4 and IL-13 Induce Chemotaxis of Human Foreskin Fibroblasts, But Not Human Fetal Lung Fibroblasts |
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Authors: | Tadashi Kohyama Xiangde Liu Fu-Qiang Wen Tetsu Kobayashi Shinji Abe Stephen I. Rennard |
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Affiliation: | Department of Respiratory Medicine, University of Tokyo, Tokyo, Japan. |
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Abstract: | Through shared receptors, IL-4 and IL-13 have been suggested to regulate not only inflammatory cells, but also to play a role in stimulating fibroblasts during fibrotic processes. Previous studies have shown that IL-4 is a chemoattractant for foreskin fibroblasts. The current study was designed to determine the effect of IL-4 and IL-13 on the migration of two types of fibroblasts: foreskin and human fetal lung fibroblasts (HFL-1). Using the Boyden blindwell chamber method, human foreskin or fetal lung fibroblasts (both 10(6)/mL) were placed in upper wells with various concentrations of IL-4 or IL-13 in the lower wells as chemoattractants. Both IL-4 (1 pg/mL) and IL-13 (100 pg/mL) induced foreskin fibroblast chemotaxis, up to 50 +/- 8 and 24 +/- 7 cells/5 high-power fields, respectively (both p < 0.05). In contrast, neither cytokine induced migration of the lung fibroblasts although both type of cells express IL-4 receptor and IL-13alpha1 receptor. These results suggest that fibroblasts are heterogeneous with regard to their ability to respond to cytokine-driven chemotaxis. Therefore, the role of specific cytokines in mediating fibrotic responses might vary depending on local mesenchymal cell responses. |
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Keywords: | airway remodeling chemotaxis fibroblasts Interleukin-4 (IL-4) IL-13 |
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