富氢林格液减轻严重烧伤大鼠延迟复苏引起的肠损伤

目的 观察富氢林格氏液（富氢液）对延迟复苏严重烧伤大鼠肠组织的保护作用。方法 制备富氢液，采用随机数字表法将36只SD大鼠随机分为3组（每组12只）即假烫组、烫伤+普通林格氏液组和烫伤+富氢液组，在普通林格氏液组和富氢液组大鼠背部造成占体表面积（TBSA） 30% 的 Ⅲ度烫伤，普通林格氏液组和富氢液组大鼠于烫伤后7h、9h、17h，分别经腹腔补给相当于总补液体积1/2、1/4、1/4的普通林格氏液或者富氢液，假烫组不补液。24h后取小肠做HE染色，检测氧化指标MDA值、MPO和SOD活性，以及促炎细胞因子IL-1β 和 TNF-α的含量。结果 相对于普通林格氏液组，富氢液组MDA值低（P<0.05），MPO和SOD活性均低（P<0.01）；促炎细胞因子IL-1β 和 TNF-α含量在富氢液组低于普通林格氏液组（P<0.01）。结论 富氢液对烧伤延迟复苏后肠组织的保护作用主要从两方面来体现，一是降低大面积烧伤及延迟复苏所带来的氧化损伤。二是抑制延迟复苏大鼠肠组织中所产生的促炎因子IL-1β和TNF-α从而减轻肠损伤。

The Protective Effect of Hydrogen-Lactated Ringer`s solution on intestine-induced by Extensive Burn in rats after delayed fluid resuscitation

Objective To investigate the protective effect of hydrogen-rich lactated Ringer’s solution(HRS) against intestine injury induced by extensive burn in Sprague-Dewley(SD) rat model after delayed fluid resuscitation. Methods Thirty-six male SD rats were randomly divided into three groups(n=12): sham-burn group(S group),burn plus normal lactated Ringer’s solution(NRS) group(BR group) and burn plus hydrogen-rich lactated Ringer’s solution(HRS) group(BH group).Rats in the S group were immersed into 37℃ water without fluid replacement;those in BR group and BH group were subjected to 30% total body surface area(TBSA) Ⅲ degree full-thickness scald.At first,a total of 2 ml·(1% TBSA)-1·kg-1 of HRS or NRS were replaced at 7 h post-burn and half volume of the total solution was replaced at the 9 h and 17 h.All rats were sacrificed at 24 h.Small intestine tissues were removed for H-E staining and determination of MDA content,MPO and SOD activity;and the levels of the IL-1β and TNF-α were determined by ELISA.Results The intestinal injury was relieved in BH group compared with BR group.The intestinal MDA content(P<0.05) and activities of MPO and SOD(P<0.01) were all decreased in BH group.IL-1β and TNF-α levels in BH group were significantly lower than those in the BR group(P<0.01).Conclusion HRS can attenuate intestine injury induced by extensive burn after delayed fluid resuscitation.It can decrease the oxidative injury following extensive burn and delay fluid resuscitation,and it can also suppress the generation of proinflammatory cytokine IL-1β and TNF-α.