唑来膦酸盐对骨髓瘤细胞的增殖及CD138表达的影响

目的 研究唑来膦酸盐对体外培养的人多发性骨髓瘤细胞KM3生长增殖及CD138表达的影响.方法 采用锥虫蓝拒染法检测1×10-5mol/L、5×10-5moL/L、1×10-4mol/L、5×10-4mol/L、1×10-3mol/L唑来膦酸盐处理后的KM3细胞存活率,用流式细胞术分析不同浓度唑来膦酸盐作用下KM3细胞凋亡率及细胞周期的变化,并进一步检测KM3细胞表面CD138表达的改变.结果 在1×10-5mol/L～1×10-3mol/L的浓度范围内,唑来膦酸盐对KM3细胞生长增殖均有抑制作用,抑制效果与唑来膦酸盐浓度呈剂量依赖性.不同浓度的唑来膦酸盐能诱导细胞凋亡,使细胞明显阻滞于S期,且呈剂量依赖趋势.在唑来膦酸盐作用下CD138+细胞数及荧光强度均降低(P值均＜0.05).结论 唑来膦酸盐对KM3细胞具有增殖抑制作用,机制可能涉及诱导凋亡、细胞周期阻滞,也可能涉及影响CD138的表达.

Effect of zoledronic acid on the growth and CD138 expression of myeloma cell Fme KM3

Objective To study the inhibitory effect of zoleronie acid(ZA)on the growth and CD138 expression of myeloma cell line KM3.Methods KM3 cells were treated with different concentrations of ZA The growth of KM3 cells was measured by trypan blue dye exclusion,and the changes of apoptosis rate,cell cycle and expression of CD138 induced by ZA by flow cytometry.Results Within the concentration of 10-5～10-3mol/L.ZA obviously inhibited the growth of KM3 cells in a dose dependent manner.IBN at 10-5～10-4mol/L increased Annexin V positive rate.blocked cells at the S/G2 boundary,reduced the expression of CD138 and its fluorescence intensity.Conclusion ZA can inhibit the growth of KM3 cells in a dose-dependent lnallner and inhibited CD138 expression.The mechanism is probably related to induction cell cycle accumulation in S phase and apoptosis.