| 猪骨髓间充质干细胞超顺磁性氧化铁标记方法的实验研究 |
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| 引用本文： | 姚志峰,姚志峰,黄浙勇,解新星,张书宁,巩雪,杨姗,张少衡,黄榕翀,金航,曾蒙苏,孙爱军,邹云增,葛均波. 猪骨髓间充质干细胞超顺磁性氧化铁标记方法的实验研究[J]. 中国临床医学, 2009, 16(5): 657-661 |
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| 作者姓名： | 姚志峰 姚志峰 黄浙勇 解新星 张书宁 巩雪 杨姗 张少衡 黄榕翀 金航 曾蒙苏 孙爱军 邹云增 葛均波 |
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| 作者单位： | 1. 复旦大学附属中山医院心内科,上海市心血管病研究所,上海,200032
2. 复旦大学附属中山医院放射科,上海,200032 |
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| 基金项目： | “十五”国家科技攻关计划项目(2004BA714B05-2);;上海市科委优秀学科带头人计划(03XD14010) |
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| 摘 要： | 目的：探索超顺磁性氧化铁纳米颗粒（SPIO）标记猪骨髓间充质干细胞（MSCs）的合适方案。方法：分离培养猪MSCs,用不同SPIO浓度、不同共孵育时间进行标记,根据标记效率指标（普鲁士蓝染色、细胞铁浓度测定、细胞透射电镜检查）和标记毒性指标（台盼蓝排除试验、CCK-8试验）确定适宜的标记条件,然后对不同标记细胞进行磁共振（MR）体外成像。结果：（1）随着SPIO浓度的增加,MSCs细胞内平均含铁量相应升高;细胞涂片普鲁士蓝染色也显示细胞内蓝染颗粒相应增加。当SPIO浓度为25、50μg·mL-1时,细胞存活率和细胞活力与无SPIO时差异无统计学意义（P〉0.05）;但当SPIO浓度增加到100μg·mL-1时,CCK-8试验显示细胞活力下降（P〈0.01）;当SPIO浓度增加到200μg·mL-1时,细胞存活率和细胞活力均下降（P〈0.05）;（2）设定SPIO浓度为50μg.mL-1,随共孵育时间增加,细胞内含铁量逐渐升高（各组间比较均P〈0.01）;当标记时间为0、2、4、8、16、24h时,细胞存活率和细胞活力并差异无统计学意义（P〉0.05）;但当标记时间进一步延长到48h时,细胞存活率有所降低（P〈0.05）;（3）按＂50μg/ml×24h方案＂标记后,1.5TMR成像仪体外MRT2＊ WIflash检测阈值为7.5×104个细胞。结论：50μg·mL-1×24h方案是猪骨髓间充质干细胞比较合适的磁探针标记方案。
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| 关 键 词： | 超顺磁性氧化铁 间充质干细胞 标记 |
The Experimental Study of Superparamagnetic Iron Oxides-Labeled Swine Bone Marrow Mesenchymal Stem Cells in vitro and the Effect on Magnetic Resonance Imaging Signals |
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| YAO Zhifeng,YAO Zhifeng,HUANG Zheyong,XIE Xinxing,ZHANG Shuning,GONG Xue,YANG Shan,ZHANG Shaoheng,HUANG Rongchong,JIN Hang,ZENG Mengsu,SUN Aijun,ZOU Yunzeng,GE Junbo. The Experimental Study of Superparamagnetic Iron Oxides-Labeled Swine Bone Marrow Mesenchymal Stem Cells in vitro and the Effect on Magnetic Resonance Imaging Signals[J]. Chinese Journal Of Clinical Medicine, 2009, 16(5): 657-661 |
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| Authors: | YAO Zhifeng YAO Zhifeng HUANG Zheyong XIE Xinxing ZHANG Shuning GONG Xue YANG Shan ZHANG Shaoheng HUANG Rongchong JIN Hang ZENG Mengsu SUN Aijun ZOU Yunzeng GE Junbo |
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| Affiliation: | YAO Zhifeng1 HUANG Zheyong1 XIE Xinxing1 ZHANG Shuning1 GONG Xue1 YANG Shan2 ZHANG Shaoheng1 HUANG Rongchong1 JIN Hang2 ZENG Mengsu2 SUN Aijun1 ZOU Yunzeng1 GE Junbo1 1. Department of Cardiology,Zhongshan Hospital,Fudan University,Shanghai Institute of Cardiovascular Diseases,Shanghai 200032,China,2. Department of Radiology |
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| Abstract: | Objective:To explore the optimal labeling procedure on swine bone marrow mesenchymal stem cells(MSCs) with superparamagnetic iron oxides(SPIO) in vitro and its effect on magnetic resonance(MR) imaging signals.Methods:MSCs isolated from swine were incubated with SPIO nanoparticles on different concentrations and times. The location,distribution and the labeling ratio of SPIO particles in MSCs was assessed by Prussian Blue staining,electron microscopy and atomic absorption spectrometry. Cell viability was obs... |
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| Keywords: | Superparamagnetic iron oxides Mesenchymal stem cells Labeling |
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