| 斑点-ELISA和独特型/抗独特型抑制试验检测日本血吸虫病患者循环表膜抗原 |
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| 引用本文： | 裘丽姝,薛海筹,张永红,李浩,诸陈文.斑点-ELISA和独特型/抗独特型抑制试验检测日本血吸虫病患者循环表膜抗原[J].中国寄生虫学与寄生虫病杂志,1991(4). |
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| 作者姓名： | 裘丽姝 薛海筹 张永红 李浩 诸陈文 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所
(裘丽姝，薛海筹，张永红，李浩)，中国预防医学科学院寄生虫病研究所(诸陈文) |
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| 摘 要： | 将血吸虫成虫表膜机原的单克隆抗体8SE4联接辣根过氧化物酶制成结合物,用于直接法斑点-ELISA检测循环抗原。结果48例血吸虫病患者的阳性率为81.3％(39例),24例正常人未出现假阳性反应。检测华支睾吸虫及并殖吸虫病患者各18例,均无交叉反应。本结果证实日本血吸虫病患者血清中有循环表膜抗原存在。直接法斑点-ELISA方法简便,具有较高的敏感性和特异性,可作为诊断日本血吸虫病的补充方法。本文还报告了独特型/抗独特型(8SE_4/抗8SE_4)抑制试验检测循环表膜抗原的初步结果。
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| 关 键 词： | 日本血吸虫病 循环表膜抗原 单克隆抗体 独特型/抗独特型抑制试验 斑点-ELISA |
DETECTION OF CIRCULATING MEMBRANE ANTIGEN OF SCHISTOSOME IN SCHISTOSOMIASIS BY DOT-ELISA AND IDIOTYPE/ANTIIDIOTYPE INTERACTION INHIBITION TEST |
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| Qiu Li-Shu,Xue Hai-Chou,Zhang Yong-Hong,Li Hao,Zhu Chen-Wen Institute of Parasitic Diseases . Chinese Academy of Preventive Medicine.DETECTION OF CIRCULATING MEMBRANE ANTIGEN OF SCHISTOSOME IN SCHISTOSOMIASIS BY DOT-ELISA AND IDIOTYPE/ANTIIDIOTYPE INTERACTION INHIBITION TEST[J].Chinese Journal of Parasitology and Parasitic Diseases,1991(4). |
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| Authors: | Qiu Li-Shu Xue Hai-Chou Zhang Yong-Hong Li Hao Zhu Chen-Wen Institute of Parasitic Diseases Chinese Academy of Preventive Medicine |
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| Abstract: | A monoclonal antibody (designated 8SE) against membrane antigen of adult Schistosomo japonicum labeled with HRP was used in dot-ELISA (direct method) to detect schistosomal circulating membrane antigen. Sera from 48 parasitologically confirmed schistosomiasis cases were tested, 39(81.396) were positive. No false positive reaction was found in sera from 24 healthy controls. No cross reaction was detected in sera from clonorchiasis or paragoni-miasis in 18 cases each. This results suggest that circulating membrane antigen does exist in patients with schistosomiasis and it might be used as a complementary method for diagnosis of schistosomiasis. A preliminary result of idiotype/antiidiotype interaction inhibition reaction for detecting circulating membrane antigen was also presented. |
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| Keywords: | Schistosomiasis circulating surface membrane antigen monoclonal antibody idiotype/ antiidiotype interaction inhibition test dot-ELISA |
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