增殖细胞核抗原与bc1-2在培养的人视网膜色素上皮细胞的表达

目的观察培养的人视网膜色素上皮细胞（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉａｌｃｅｌｓ，ＲＰＥ）的增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｖｅｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）和ｂｃ１－２的表达。方法用ＳＡＢＣ法对培养的人ＲＰＥ作鼠抗人ＰＣＮＡ单抗及兔抗人ｂｃ１－２抗体免疫组织化学染色。结果ＲＰＥ在接种后２４小时和４８小时，ＰＣＮＡ的阳性率分别为３１．２％和５０．６％，阳性染色位于细胞核内，斑块状。ｂｃ１－２的表达为７６％～９０％，阳性染色在细胞浆，细颗粒状。结论培养中一半ＲＰＥ有ＰＣＮＡ抗原表达，提示这些细胞处于Ｓ期；ｂｃ１－２抗原在大多数ＲＰＥ表达阳性。这些生物标记物可能与培养的ＲＰＥ生长活性有关。

Expression of PCNA and bcl 2 of cultured human retinal pigment epithelial cells

Purpose To observe the expression of proliferating cell nuclear antigen(PCNA)and bcl 2 of cultured human retinal pigment epithelial cells(RPE). Methods SABC techniques were applied for immunocytochemical staining of cultured RPE with mouse anti human PCNA monoclonal antibody and rabbit anti human bcl 2 antibodies. Results 31.2% and 50.6% cultured cells were positive to anti human PCNA at 24h and 48h after seeding,respectively.The positive staining was mottled in the nucleus.Positive staining for bcl 2 was seen in 76%to 90% cells as fine granules scattered within the cytoplasm. Conclusion One half of cultured RPE expressed PCNA,indicating that the cells were in phase S of the cell cycle.Positive staining for bcl 2 appeared in much more RPE cells.These biological markers may be associated with the growth activity of cultured RPE.