麝香合剂对大鼠急性心肌梗死的影响(英文)

目的:观察麝香合剂对急性心肌梗死大鼠梗死范围、血清肌酸磷酸激酶(CPK)和乳酸脱氢酶(LDH)活力以及血流动力学的影响。方法:雄性SD大鼠随机分为6组,假手术组,心肌梗死组,麝香合剂3.8,7.6,15.2pill·kg-1组和麝香保心丸0.6pill·kg-1组,分别灌胃给予0.5%羧甲纤维素钠(CMCNa),0.5%CMCNa,3种浓度麝香合剂混悬液,麝香保心丸混悬液,每日给药1次,连续给药3d,给药容积为10mL·kg-1。最后1次给药后30min,结扎冠状动脉左前降支(LAD)制备心肌梗死模型。冠状动脉结扎后24h,测量大鼠的梗死范围、血清CPK和LDH活力以及血流动力学参数。结果:麝香合剂7.6,15.2pill·kg-1组和麝香保心丸组大鼠的梗死范围分别是(15.2±2.4)%,(13.±2.0)%和(18±3)%,均显著小于心肌梗死组大鼠(21.9±2.0)%,P<0.01]。心肌梗死组大鼠的血清CPK和LDH活力均明显高于假手术组(P<0.05,P<0.01),而升高的酶活力可以被预先给予麝香合剂或麝香保心丸所降低(P<0.05P<0.01)。心肌梗死组大鼠的动脉收缩压(SBP)动脉舒张压(DBP)、左室收缩压峰值(Peak)、左室压最大上升速率/下降速率(±dp/dtmax)和心肌最大缩短速率(Vmax)明显降低(P<0.05,P<0.01),而左室舒张末期压(LVEDP)明显升高(P<0.01)。预先给予麝香合剂7.6,15.2pill·kg-1和麝香保心丸可使降低的+d

Effects of Shexiang mixture in a rat model of acute myocardial infarction

AIM: To observe the effects of Shexiang mixture(SXM) on the infarct size, serum creatine phosphokinase(CPK) and lactic dehydrogenase (LDH) activities and hemodynamics in a rat model of acute myocardial infarction. METHODS: Male Sprague-Dawley(SD)rats were randomly divided into six groups, namely sham-operated group(sham group), myocardial infarction group(MI group), SXM 3.8, 7.6, 15.2 pill·kg-1 groups, and Shexiang Baoxin Wan (SXBXW) 0.6 pill·kg-1 group. They were daily administered intragastrically with 0.5 % carboxy-methylcellulose-Na(CMC-Na), 0.5 % CMC-Na, three concentrations of SXM suspension (38, 76, 152 pills·100 mL-1 of 0.5 % CMC-Na, respectively), and SXBXW suspension (6 pills·100 mL-1 of 0.5 % CMC-Na) for 3 d, respectively. The volume of giving drugs was 10 mL ·kg-1. Thirty minutes after last administration, MI was surgically induced by ligation of the left anterior descending coronary artery (LAD). Infarct size, serum CPK and LDH activities, and hemodynamic parameters were measured 24 h after the ligation of the LAD. RESULTS: The infarct sizes of SXM 7.6, 15.2 pill·kg-1 groups and SXBXW group were (15.2±s 2.4) %, (13.5±2.0) % and (18±3) %, respectively, all of them were significantly smaller than that of MI group ((21.9±2.0) %, P <0.01)). In MI group, serum CPK and LDH activities were remarkably higher than those in sham group(P<0.05, P<0.01). The increased serum CPK and LDH activities induced by ligation of the coronary artery were significantly reduced by pretreatment with SXM and SXBXW(P<0.05, P<0.01). In MI rats, systolic blood pressure(SBP), diastolic blood pressure(DBP), peak systolic left ventricular pressure(Peak), maximum rate of left ventricular pressure rise(+dp/dtmax), maximum rate of left ventricular pressure decline (-dp/dtmax), myocardial maximal shortening velocity(Vmax) were remarkably decreased(P <0.05, P <0.01), but left ventricular end diastolic pressure (LVEDP) was significantly increased than those in sham group(P <0.01). However, reduced +dp/dtmax and Vmax could be increased, and elevated LVEDP could be decreased by pretreatment with SXM 7.6, 15.2 pill·kg-1 and SXBXW, significantly(P <0.05, P <0.01). Furthermore, the fall in SBP and -dp/dtmax could be prevented by SXM 7.6, 15.2 pill·kg-1(P <0.05) rather than SXBXW(P >0.05). CONCLUSION: Same as SXBXW, SXM could reduce infarct size, decrease serum CPK and LDH activities, and improve cardiac function in a rat model of acute myocardial infarction.