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金银花及其提取物指纹图谱研究
引用本文:冯文宇,彭峰,何兵,邱华荣,田吉.金银花及其提取物指纹图谱研究[J].泸州医学院学报,2003,26(4):283-286.
作者姓名:冯文宇  彭峰  何兵  邱华荣  田吉
作者单位:1. 泸州医学院药学院
2. 泸州市卫生防疫站,四川,泸州,646000
摘    要:目的:研究建立金银花及金银花提取物指纹图谱检测标准。方法:以绿原酸为参照物,甲醇-0.4%磷酸溶液(38:62)为流动相,应用液相色谱在波长325nm下,分别检测各批样品的指纹图。结果:药材、提取物均有6个共有峰,共有峰的参数符合“中药注射剂指纹图谱技术要求”的有关规定。结论:方法准确,重复性好,建立的指纹图谱检测标准,可作为金银花及其提取物质量评价与品种鉴别的重要依据之一。

关 键 词:金银花  金银花提取物  液相色谱  指纹图谱
文章编号:1000-2669(2003)04-0283-04
修稿时间:2003年4月20日

STUDY ON FINGERPRINT OF FLOS LONICERAE AND ITS EXTRACT
Feng Wenyu,et al Pharmacy College of Luzhou Medical College.STUDY ON FINGERPRINT OF FLOS LONICERAE AND ITS EXTRACT[J].Journal of Luzhou Medical College,2003,26(4):283-286.
Authors:Feng Wenyu  Pharmacy College of Luzhou Medical College
Abstract:Objective:To establish the fingerprint detecting standard of Jinyinhua Flos Lonicerae and its extract. Methods: Chlorogenic acid was used as the reference substance and methanol - 0.4% phosphoric acid solution (38:62) as mobile phase. The fingerprint of separate batch of samples was detected with HPLC, under wavelength 325nm. Results :The crude drug or extract all had 6 peaks, the parameter of which met with the stipulation of Chinese medicine injection fingerprint technical requirements. Conclusion:The method is simple and accurate with a good reproducibility and can be used in quality evaluation and variety discrimination of Jinyinhua (Flos Lonicerae) and its extract.
Keywords:Flos lonicerae  Jinyinhua  Jinyinhua extract  HPLC  Fingerprint
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