| Z24对大鼠肝脏CYP450酶系的影响 |
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| 引用本文： | 王莹,王全军,吴纯启,施畅,王青秀,廖明阳. Z24对大鼠肝脏CYP450酶系的影响[J]. 中国新药杂志, 2008, 17(19): 1668-1670 |
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| 作者姓名： | 王莹 王全军 吴纯启 施畅 王青秀 廖明阳 |
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| 作者单位： | 1. 辽宁医学院药物研究所,辽宁,锦州,121002;军事医学科学院毒物药物研究所,国家北京药物安全评价研究中心,北京,100850
2. 军事医学科学院毒物药物研究所,国家北京药物安全评价研究中心,北京,100850 |
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| 摘 要： | 目的:观察Z24对大鼠肝脏CYP450酶系的影响.方法:雌性Wistar大鼠,灌胃(ig)给予Z24(0,50,100,200 mg·kg-1·d-1),连续5 d,以0.9%氯化钠溶液作对照,末次给药后次日,处死大鼠,测定肝微粒体中CYP450总含量、细胞色素b5(Cyt-b5)含量、NADPH-CYP450还原酶活性以及1A2,181,2E1,3A4亚型活性.结果:与对照组相比,各剂量组CYP450总含量、NADPH-CYP450还原酶活性及CYPlB1、2El亚型活性均明显升高(P＜0.05);100和200 mg·kg-1组Cyt-b5含量升高(P＜0.05);200 mg·kg-1组CYPlA2和3A亚型活性升高(P＜0.05).结论:Z24对CYP450、NADPH-CYP450还原酶及CYPlB1、2El亚型有诱导作用,剂量达100和200 mg·kg-1时分别对cyt-b5和CYPlA2、3A亚型产生诱导作用.
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| 关 键 词： | 肝脏 细胞色素P450 诱导作用 |
Effects of Z24 on rat liver cytochrome P450 system |
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| WANG Ying,WANG Quan-jun,WU Chun-qi,SHI Chang,WANG Qing-xiu,LIAO Ming-yang. Effects of Z24 on rat liver cytochrome P450 system[J]. Chinese Journal of New Drugs, 2008, 17(19): 1668-1670 |
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| Authors: | WANG Ying WANG Quan-jun WU Chun-qi SHI Chang WANG Qing-xiu LIAO Ming-yang |
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| Affiliation: | 1 Institute of Pharmacology;Liaoning Medical College;Jinzhou 121002;China;2 Institute of Pharmacology and Toxicology;Academy of Military Medical Sciences;National Beijing Center for Drug Safety Evaluation and Research;Beijing 100850;China |
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| Abstract: | Objective: To investigate the effect of Z24 on the contents and activities of cytochrome P450 (CYP450) system in rat liver. Methods: In female Wistar rats, Z24 (50, 100 and 200 mg•kg-1) or saline (control) was intragastrically administrated for 5 days. Then, total contents of CYP450 and cytochrome b5, and activities of NADPH-CYP450 reductase and the subtypes CYP1A2,1B1,2E1,3A in liver microsome were detected. Results: Total CYP450 contents, activities of NADPH-CYP450 reductase and subtype CYP1B1, 2E1 were higher in all three Z24 groups than in control group. Cytochrome b5 content increased in Z24 100 and 200 mg•kg-1 groups (P<0.05), and the activities of subtypes CYP1A2 and CYP 3A increased in Z24 200 mg•kg-1 group (P<0.05). Conclusion: CYP450, NADPH-CYP450 reductase and subtypes CYP1B1, 2E1 can be induced by Z24. Cytochrome b5, and subtypes 1A2 and 3A can also be induced by Z24 at 100 and 200 mg•kg-1. |
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| Keywords: | Z24 |
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