透明质酸载碱性成纤维细胞生长因子对兔膝关节缺血再灌注损伤防治作用的实验研究

目的：通过动物实验观察碱性成纤维细胞生长因子(bFGF)与透明质酸(HA)关节腔内注射对关节缺血再灌注损伤及退行性关节炎的防治作用。方法：采用夹闭兔股血管8h的方法模拟缺血再灌注模型。将48只新西兰大白兔随机分为对照组(A组)，HA治疗组(B组)和FGF／HA治疗组(C组)，在关节腔内注射相应的药物。用光镜和电镜观察关节滑膜和软骨的病理改变，观察并半定量计算软骨蛋白多糖(PGs)的变化，检测滑膜丙二醛(MDA)的含量。结果：C组的软骨和滑膜病理改变明显轻于A组和B组，在C组中，滑膜MDA的含量明显低于其它两组，而PGs的含量明显高于其它两组：结论：联合应用bFGF和HA能明显减轻兔膝关节缺血再灌注损伤和退行性改变，其作用要明显优于单用HA。

An experimental study of the effects of basic fibroblast growth factor and hyaluronic acid on the rabbit's knee joint injury after ischemia-reperfusion

Objective:To investigate the effect of intraarticular basic fibroblast growth factor (bFGF) and Hyaluronic acid (HA) injection for the prevention of the ischemic-reperfusion injury and the degenerative change of articular cartilage and synovium after the rabbit's knee joint ischemia-reperfusion.Method:The rabbit's hind limb fermoral arteries were clapped for eight hours and then loosen to establish the animal ischemia-reperfusion model of the knee joint.A total of 48 healthy adult rabbits were randomly divided into three groups:the control group (group A):HA injection group (group B);bFGF/'HA injection group (group C).Corresponding medicines were injected into Ischemia-reperfusien knee joint cavity respectively,Pathologic changes of articular cartilage and synovium were observed with the light microscope and transmission electron microscope.Cartilage Proteoglycans (PGs) changes were observed and analysed semiquantitively.Malonic diadehyde (MDA) content of synovium was determined.Result:The improvement of the histological structure of group C was better than group A and group B.PGs content of group C was higher than the other two groups.MDA content of group C was lower than the other two groups.Conclusion:bFGF/HA injection into knee joint cavity can alleviate the ischemic-reperfusion injury and the degenerative change of articular cartilage and synovium after thc rabbit's knee joint ischemia-reperfusion.