不育男性精浆总抗氧化能力与精子运动功能的关系

目的:研究不育男性精浆总抗氧化能力(TAC)与精子运动能力和方式之间的关系,探讨精浆TAC水平在男性生育中的临床意义。方法:113例精子密度正常的不育男性,28例正常生育男性作为对照组。精液于37℃液化后采用计算机辅助精液分析(CASA)系统进行精液常规分析,采用比色法进行精浆TAC分析。结果:正常生育组精浆TAC为(19.82±6.33)U,不育男性精子密度正常组精浆TAC为(14.37±8.45)U,不育男性精子密度正常组与正常生育组比较存在显著性差异(P<0.01)。精浆TAC与a级精子百分率(r=0.208,P<0.05)和(a+b)级精子百分率(r=0.231,P<0.05)呈显著正相关,精浆TAC与精子运动参数中的前向性(r=0.200,P<0.05)、直线性(r=0.208,P<0.05)、曲线速度(r=0.189,P<0.05)、直线速度(r=0.210,P<0.05)、平均移动速度(r=0.215,P<0.05)及鞭打频率(r=-0.248,P<0.01)之间有显著的相关性,其中前向性、直线性、直线速度、曲线速度、平均移动速度与TAC呈正相关(P<0.05),而鞭打频率与TAC呈负相关(P<0.01)。精浆TAC与摆动性、侧摆幅度、平均移动角度之间无显著相关。结论:精浆中TAC水平与精子运动能力和运动方式密切相关,适宜的精浆TAC为精子运动提供了良好的外部环境,精浆中过低的TAC水平与精子运动能力下降和运动方式改变有关,可能是引起男性不育的病因之一。精浆中TAC分析可为探讨男性不育的发病机制以及临床用药提供依据。

Correlation of Total Antioxidant Capacity in Seminal Plasma with Sperm Motility of Infertile Men

OBJECTIVE: To evaluate the correlation of total antioxidant capacity (TAC) in seminal plasma with sperm motility in infertile men, and explore the clinical significance of TAC in seminal plasma in male fertility. METHODS: One hundred and thirteen infertile men with normal sperm density were included in the experiment group and 28 fertile men in the normal control. The seminal parameter analysis was performed by computer-assisted semen analysis (CASA) system. Seminal plasma TAC was measured with spectroscopic analysis. RESULTS: Seminal plasma TAC was (14.37 +/- 8.45) U in the infertile men with normal sperm density and (19.82 +/- 6.33) U in the fertile control. Compared with the fertile men, seminal plasma TAC in the experiment group was significantly lower (P < 0.01). There was significant correlation between seminal plasma TAC and sperm motility, grade a sperm (r = 0.208, P < 0.05), grade (a + b) sperm (r = 0.231, P < 0.05), straightness (STR) (r = 0.200, P < 0.05), linearity (LIN) (r = 0.208, P < 0.05), curvilinear velocity (VCL) (r = 0.189, P < 0.05), straight line velocity (VSL) (r = 0.210, P < 0.05), average path velocity (VAP) (r = 0.215, P < 0.05), and beat cross frequency (BCF) (r = -0.248, P < 0.01). There was no significant correlation among the average motion degree (MAD), the amplitude of lateral head displacement (ALH) and wobbly (WOB). CONCLUSION: TAC in seminal plasma is closely related to male fertility, appropriate TAC provides a favourable environment for sperm swimming. The decreased level of TAC in seminal plasma may be one of the causes of male infertility. The analysis of TAC in seminal plasma may afford valuable evidence in exploring the mechanism of male infertility and in clinical medication.