| 抗RSV人-鼠嵌合抗体的构建及表达 |
| |
| 引用本文： | 黄晋生,段佩若.抗RSV人-鼠嵌合抗体的构建及表达[J].现代免疫学,1999,19(6):329-333. |
| |
| 作者姓名： | 黄晋生 段佩若 |
| |
| 作者单位： | 解放军海军总医院!北京100037(黄晋生，张海荣，乔媛媛，段佩若)，解放军262医院!北京100088(陆柔剑)，中国预防医学科学院病毒研究所!北京100052(王琰) |
| |
| 基金项目： | 国家863 计划资肋课题!(863 102 09 02 01) |
| |
| 摘 要： | 用RT PCR法克隆抗RSV 鼠单抗4C2 的轻、重链可变区基因, 构建了轻、重链分别表达的载体pAG4622_4C2 和pAH4604 4C2, 及轻、重链同时表达的载体pdHL4C2, 分别转染SP2/0 细胞和DHFR- CHO细胞, 检测培养上清中的嵌合抗体表达。结果在pdHL4C2 转染的CHO 细胞中检测出了可与表达RSVG/F糖蛋白的143TK 细胞结合的人 鼠嵌合抗体
|
| 关 键 词： | 呼吸道合胞病毒 免疫球蛋白基因 人-鼠嵌合抗体 |
Construction and Expression of 4C2 Human Mouse Chimeric Antibody Directed Against RSV |
| |
| Huang Jinsheng,Zhang Hairong,Qiao Yuanyuan,Duan Peiruo,Lu Roujian,Wang Yan.Construction and Expression of 4C2 Human Mouse Chimeric Antibody Directed Against RSV[J].Current Immunology,1999,19(6):329-333. |
| |
| Authors: | Huang Jinsheng Zhang Hairong Qiao Yuanyuan Duan Peiruo Lu Roujian Wang Yan |
| |
| Abstract: | An anti RSV human mouse chimeric antibody was constructed,firstly by cloning the light and heavy chain of the mouse anti RSV monoclonal antibodies 4C2 by RT PCR,and then constructing the light chain expressing vector pAG4622 4C2,heavy chain expressing vector pAH4604 4C2,as well as the simultaneously expressing vector pdHL4C2 Transfections of SP2/0 and DHFR - CHO cells with these constructed genes were conducted respectively It was found that the human mouse chimeric antibodies that could bind with the RSV G/F glycoprotein might be detected in the supernatant of pdHL4C2 transfected CHO cells |
| |
| Keywords: | RSV immunoglobulin genes human mouse chimeric antibody |
| 本文献已被 CNKI 维普 等数据库收录！ |
