血管内皮生长因子在乳腺癌中的表达及与微血管生成的关系

目的 :探讨血管内皮生长因子 (VEGF)在乳腺癌中的表达及其与血管生成和病理参数的关系。方法 :采用S P免疫组织化学染色法检测 5 6例乳腺癌及 10例癌旁正常组织的VEGF表达 ,并对血管进行Ⅷ因子相关抗原的免疫组织化学染色 ,记数微血管密度。对 10例乳腺癌进行VEGFmRNA原位杂交检测。结果 :乳腺癌组织VEGF表达阳性率高于其癌旁正常组织 ,差异有显著意义 ,P <0 0 5。VEGF的表达与乳腺癌的病理分级密切相关 ,P <0 0 1。有淋巴结转移的VEGF表达率明显高于无淋巴结转移 ,P <0 0 5 ;而且随着VEGF表达的增强 ,乳腺癌组织内微血管密度明显增高 ,P <0 0 1。结论 :癌组织VEGF表达增强在肿瘤血管生成、生长及转移中起重要作用。

Expression of VEGF and the Relationship Between It and MVD in Breast Carcinoma

Objective To clarify the relationship between the expression of vascular endothelial growth factor(VEGF) and the angiogenesis,pathological factors in breast carcinoma.Methods The S-P immunohistochemistry and VEGFmRNA situ hybridization were used.Results The positive rate of VEGF in breast carcinoma was higher than that in normal tissue,P<0.05.The expression of VEGF was closely related to pathological grade and lymphatic metastasis,P<0.01.P<0.05,respectively.A significantly higher microvessel density(MVD) was observed in the breast carcinoma with positive expression of VEGF compared with that of the negative expression,P<0.01.Conclusions The up-regulation of VEGF has an important role in angiogenesis,growth and metastases of breast carcinoma.