Changes in Idiotypic Predominance in the Anti-Arsonate Response by Priming with Anti-Idiotypic Antibodies

The predominant selection of CRI-A-bearing antibodies during the anti-arsonate (ARS) response of A/J mice has been used as a model to analyse the mechanism involved in the process of clonal selection and establishment of predominance. In order to assess the importance of the affinity and adaptability of CRI-A clones in this process, we tested the capability of a minor recurrent idiotype (id-1A3), present in a CRI-Aanti-ARS monoclonal antibody (65-1A3), to develop a normal anti-ARS response. Our results show that the id-1A3 predominance, established by anti-id-1A3 administration was stable during the primary and secondary anti-ARS response and that this predominance occurred concomitantly with low levels of CRI-A. Thus, a change in the idiotype predominance was achieved. In spite of the high levels of id-1A3, the anti-ARS antibody concentration, the affinity values, and the kinetics of the immune response were similar to those of the control group. All these results show that CRI-A clones are not essential in the normal development of the anti-ARS antibody response of A/J mice, and suggest that factors other than affinity could be involved in the establishment of the CRI-A predominance.