Effect of Ureaplasma urealyticum infection on IL-laand IL-6 secretion by rat Sertoli cells

Objective: To observe the effect of Ureaplasma urealyticum (UU) infection on the IL-1α and IL-6 secretion by rat Sertoli cells. Methods: Eight 20-day-old UU-free male SD rats (average weight 40 g) were used. Under sterile condition, the testes were removed and separately digested with collagenase typeⅡand hyaluronidase. High purity Sertoli cells were then isolated and adjusted to a concentration of 8×105/mL with DMEM/Ham's F-12. In the infected group, 1 mL Sertoli cell suspension and 100 mL UU (serotype 8, T960) were introduced into one well of a 24 well culture plate. In the control group, 1 mL Sertoli cell suspension and 100 mL medium were introduced. IL-1αand IL-6 were determined in the culture supernatant with ELISA. Results: The production of IL-1αwas significantly lower and of IL-6 significantly higher in the infected than those in the control groups (P<0.01). Conclusion: UU infection reduces the IL-1αand increases the IL-6 secretion by rat Sertoli cells. UU infection is probably involved in

Effect of Ureaplasma urealyticum infection on IL-laand IL-6 secretion by rat Sertoli cells</b></span></td> </tr> <tr> <td align="left" height="15" valign="top"></td><td valign="top" align="left"></td> </tr> <tr> <td align="left" height="25" valign="top" colspan=2"><author>Mei-Ge LU,Jian-Li SHI,Chen XU. <title>Effect of Ureaplasma urealyticum infection on IL-laand IL-6 secretion by rat Sertoli cells[J]. Asian journal of andrology, 2003, 0(2)</td> </tr> <tr> <td align="left" height="10" valign="top"></td><td valign="top" align="left"></td> </tr> <tr> <td align="left" height="25" valign="top" nowrap><b>Authors:</b></td><td width="92%" valign="top" align="left" ><a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=%3cauthor%3eMei-Ge+LU&prev_q=%3cauthor%3eMei-Ge+LU'><author>Mei-Ge LU</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Jian-Li+SHI&prev_q=Jian-Li+SHI'>Jian-Li SHI</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Chen+XU&prev_q=Chen+XU'>Chen XU</a></td> </tr> <tr><td align="left" height="10" valign="top"></td><td valign="top" align="left"></td></tr> <tr> <td align="left" height="25" valign="top" nowrap><b>Affiliation:</b></td><td width="92%" valign="top" align="left" ><author>Mei-Ge LU,Jian-Li SHI,Chen XUDepartment of Histology & Embryology,Shanghai SecondMedical University,Shanghai 200025,China</td> </tr> <tr><td align="left" height="10" valign="top"></td><td valign="top" align="left"></td></tr> <tr> <td align="left" height="25" valign="top" nowrap><b>Abstract:</b></td><td width="92%" align="left" valign="top"></td> </tr> <tr> <td align="left" height="15" valign="top"></td><td valign="top" align="left"></td> </tr> <tr> <td align="left" valign="top" height="25" nowrap><b>Keywords:</b></td><td width="92%" valign="top" align="left" ></td> </tr> <tr> <td align="left" colspan="2" height="30"><b>本文献已被 <a href="http://www.cnki.com.cn/Article/CJFDTotal-YZNK200302022.htm" target="_blank">CNKI</a> 等数据库收录！</b></td> </tr> </table> </td> </tr> </table> <table width="870" align="center"> <tr><td align="left"> <div id="WasReferencedList"> </div> </td></tr> <tr><td align="left"> <div id="RelateArticleList"> </div> </td></tr> </table> <table width="870" border="0" align="center" cellpadding="0" cellspacing="0"> <tr> <td height="40" align="center" class="fo2"> <a href="#" onClick="this.style.behavior='url(#default#homepage)';this.setHomePage('http://yyws.alljournals.cn')">设为首页</a> <span class="STYLE1">|</span> <a href="http://www.alljournals.cn/note.aspx">免责声明</a> <span class="STYLE1">|</span> <a href="http://www.e-tiller.com"><u>关于勤云</u></a> <span class="STYLE1">|</span> <a href="javascript:window.external.addFavorite('http://yyws.alljournals.cn','期刊界 All Journals---医药、卫生')">加入收藏</a></td> </tr> <tr> <td align="center" class="fo3"> <p align="center"> Copyright<span lang="en">©<a href="http://www.e-tiller.com"><u>北京勤云科技发展有限公司</u></a> 京ICP备09084417号 </span></p> </td> </tr> </table> </body> </html> <script language=javascript> $(document).ready(function() { $("div#RelateArticleList").html("正在获取相似文献，请稍候..."); $.get("relate_article_list.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=527A01A248DACB72&aid=5026A88B135621BD0C5E148C8495EB46&language=2&ctl=90&etl=97",function(xml){ if(xml==""){ $("div#RelateArticleList").html(""); }else{ $("div#RelateArticleList").html(xml); } }); }); </script>