Effects of arsenic speciation and low dissolved oxygen condition on the toxicity of arsenic to a lotic mayfly

The influence of site-specific conditions on contaminant bioavailability and toxicity to benthic invertebrates is a key consideration in the environmental risk assessment process. This is particularly relevant for contaminants with complex speciation chemistries, such as arsenic. The present study addressed uncertainties regarding arsenic toxicity to a mayfly (Baetis tricaudatus) under low dissolved oxygen (DO) conditions characteristic of many contaminated sites. Arsenic toxicity (arsenite, As(III); arsenate, As(V)) to mayfly nymphs was assessed under two DO scenarios (68 and 84% saturation). Arsenic speciation ratios were determined during testing to confirm the nature of arsenic exposure. The present study found that As(III) was more lethal and bioaccumulated to a greater degree in B. tricaudatus compared to As(V), but the sublethal toxicities of the two arsenic species were similar. Nymph growth and development were significantly inhibited after 12 d of exposure to both 1 mg/L of As(III) and As(V). Exposure to arsenic under low DO conditions (6.5 mg/L, 68% saturation) did not significantly affect As(III) or As(V) toxicity and bioaccumulation over 12 d. The DO level of 6.5 mg/L, however, appeared to be marginally lethal to B. tricaudatus. Results indicate that the Canadian arsenic criterion for the protection of aquatic life (5 microg/L) is protective of B. tricaudatus and is low enough to accommodate differences in arsenic toxicity because of the interconversions between As(III) and As(V). These findings provide insight regarding the toxicity and speciation of arsenic under DO conditions considered to be low for this lotic mayfly species and representative of existing conditions at mine sites in northern Canada.