Relationship between detection limit and bias of accuracy of quantification of RNA by RT-PCR |
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Authors: | Urban Carsten Schweinberger Andreas Kundi Michael Dorner Friedrich Hämmerle Thomas |
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Institution: | Baxter Vaccine AG, Biomedical Research Center, Uferstr. 15, 2304, Orth/Donau, Austria. |
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Abstract: | Evidence is presented demonstrating that the distribution of data obtained applying a given RT-PCR method deviates from a normal distribution depending on the limit of detection. The effect of this is a bias towards higher values and concomitantly a systematic error in respect to the accuracy of the evaluation due to this deviation from normality. In addition, evidence is presented that an evaluation assuming a log-normal distribution is more appropriate. |
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