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| 龙牙楤木总皂苷对缺血/再灌注心肌细胞收缩功能和钙瞬变的影响 | |
| 引用本文： | 张妙笛,孙桂波,徐惠波,王敏,孙晓波.龙牙楤木总皂苷对缺血/再灌注心肌细胞收缩功能和钙瞬变的影响[J].中国中药杂志,2015,40(12):2403-2407. |
| 作者姓名： | 张妙笛 孙桂波 徐惠波 王敏 孙晓波 |
| 作者单位： | 1. 哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨,150076 2. 中国医学科学院药用植物研究所药理毒理中心,北京,100193 3. 吉林省中医药科学院,吉林长春,130021 |
| 基金项目： | 国家自然科学基金项目(81173589,81473380);北京市自然科学基金项目(7142108);高等学校博士学科点专项科研基金(20121106110033) |
| 摘 要： | 为探讨龙牙楤木总皂苷(AS)对缺血/再灌注(I/R)诱导大鼠心肌细胞损伤的保护作用,采用乳酸钠灌注液灌注成年大鼠的心室肌细胞模拟缺血,含钙台式液模拟再灌注,细胞收缩与离子浓度同步测定系统同步检测AS对单个I/R细胞收缩和钙瞬变的影响.发现AS使再灌注后心肌细胞静息态肌小节长度、收缩幅度、收缩/舒张速度以及钙瞬变幅度、速度增大(P ＜0.05,P＜0.01),细胞达到舒张时最大长度的90.0％的时间、细胞收缩达峰值的时间、稳态钙静息值、收缩期Ca2+]i上升到最高的50.0％的时间、胞内钙瞬变衰减率减少(P ＜0.05,P＜0.01).因此,推测AS改善了缺血再灌注后细胞收缩与钙稳态的损伤. |
| 关 键 词： | 龙牙楤木总皂苷 心肌细胞 缺血再灌注 细胞收缩与离子浓度同步测定系统 |
| 收稿时间： | 2014/11/6 0:00:00 |
Effect of aralosides to contraction function and calcium transient of ischemia/reperfusion myocardial cells |
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| ZHANG Miao-di,SUN Gui-bo,XU Hui-bo,WANG Min and SUN Xiao-bo.Effect of aralosides to contraction function and calcium transient of ischemia/reperfusion myocardial cells[J].China Journal of Chinese Materia Medica,2015,40(12):2403-2407. | |
| Authors: | ZHANG Miao-di SUN Gui-bo XU Hui-bo WANG Min and SUN Xiao-bo |
| Institution: | Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China,Pharmacology and Toxicology Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China,Academy of Chinese Medical Sciences of Jilin Province, Changchun 130021, China,Pharmacology and Toxicology Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China and Pharmacology and Toxicology Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China |
| Abstract: | To discuss the protective effect of aralosides (AS) on I/R-induced rat myocardial injury. The adult rat ventricular myocyte ischemia model was established through perfusion with sodium lactate perfusate and reperfusion with Ca2+-containing Tyrode's solution simulation. The cell contraction and ion concentration synchronization determination system was applied to detect the effect of AS on single I/R cell contraction and Ca2+ transients. According to the findings, AS could increase resting sarcomere length, contraction amplitude, ±dL/dtmax, calcium transient amplitude and speed of post-reperfusion myocardial cells (P<0.05, P<0.01), and decrease in time for achieving 90.0% of maximum relaxation, time for achieving peak value, resting calcium ratio, contraction period Ca2+]i, time for achieving 50.0% of maximum relaxation and attenuation rate of intracellular calcium transient (P<0.05, P<0.01). Therefore, it is suggested that AS improved the post-reperfusion cell contraction and injury of calcium homeostasis. |
| Keywords: | aralosides myocyte ischemia/reperfusion cell contraction and ion concentration synchronization determination system |