脓毒症中骨骼肌有氧糖酵解增强与细胞Na^＋,K^＋,ATP酶活性改变 …

观察和分析细胞膜有关的能量代谢状态改变对正常及脓毒症大量的不同类型骨骼肌乳酸产生量的影响。方法肛助大鼠脓毒症模型，建立伸趾长肌和比目鱼肌的充分供氧离体孵育系统，利用特异性Ｎａ＾＋、Ｋ＾＋、ＡＴＰ酶抑制剂－哇巴因，干预细胞膜钠钾泵的活性，采用ＮＡＤＨ荧光探针方法，检测骨骼肌组织细胞乳酸的产生量及其变化。

Experimental study on the enhancement of aerobic glycolysis ofmuscles in sepsis and its relationship with the activity of the intracellular Na+,K+,ATPase

Objective:To analyze and evaluate the impact of the changes in the membrane related energetic metabolism on the cellular lactate production of different muscular cells in septic rats as compared to that of the normal ones. Methods: Using a rat sepsis model, we established the in vitro muscle incubation system with sufficient oxygen supply as well as the NADH fluorometry method for the detection of trace amount of lactate in the samples. By using the specific ATPase inhibitor ouabain, the activity of Na + pump was inhibited to various degress and the lactate production of the muscle cells was measured accordingly.Results: (1) the lactate production was significantly inhibited by ouabain in muscle cells both in normal and septic rats; (2) The inhibitory effects of ouabain on aerobic glycolysis in the extensor digitorium longus (EDL) muscles were more obvious and sensitive than those in the soleus muscles. Conclusion: In normal and septic states, the activity of the Na + pump may be stimulated by several factors in relationship with cellular functional activities, which in turn play important roles in the increment of lactate production in muscle cells.