| 广泛耐药鲍曼不动杆菌对氨基糖苷类耐药机制 |
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| 引用本文： | 张丽花,蔡培泉,王春新. 广泛耐药鲍曼不动杆菌对氨基糖苷类耐药机制[J]. 中外医疗, 2013, 0(35): 10-12 |
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| 作者姓名： | 张丽花 蔡培泉 王春新 |
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| 作者单位： | [1]张家港市第一人民医院检验科,江苏张家港215600 [2]南京医科大学附属无锡人民医院,江苏无锡214023 |
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| 摘 要： | 目的 调查广泛耐药鲍曼不动杆菌（Extensively drug Resistant Acinetobacter Baumannii,XDR-ABA）临床分离菌株中氨基糖苷类修饰酶基因、16SrRNA甲基化酶基因和外排泵基因的存在情况.方法 收集2011年1-12月临床标本中分离的XDR-ABA菌20株,采用微量肉汤稀释法进行抗菌药物敏感性试验,聚合酶链反应（PCR）方法分析9种氨基糖苷类修饰酶基因、6种16SrRNA甲基化酶基因和外排泵基因adeB.结果 20株XDR-ABA菌均检出aac（6＇）-Ⅰb、ant（2＂）-Ⅰ、ant（3＂）-Ⅰ、aphA1和adeB外排泵基因,其余5种氨基糖苷类修饰酶基因和6种16SrRNA甲基化酶基因和均未检出.结论该组20株XDR-ABA菌耐多种氨基糖苷类药物与细菌产aac（6＇）-Ⅰb、ant（2＂）-Ⅰ、ant（3＂）-Ⅰ、aphA1 4种氨基糖苷类修饰酶和存在adeABC外排泵系统相关.
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| 关 键 词： | 鲍曼不动杆菌 氨基糖苷类修饰酶基因 16SrRNA甲基化酶基因 外排泵 广泛耐药 |
Study on Aminoglycoside Antibiotic Resistant Mechanism in Extensively Drug Resistant Acinetobacter Baumannii |
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| ZHANG Lihua,CAI Peiquan,WANG Chunxin. Study on Aminoglycoside Antibiotic Resistant Mechanism in Extensively Drug Resistant Acinetobacter Baumannii[J]. China Foreign Medical Treatment, 2013, 0(35): 10-12 |
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| Authors: | ZHANG Lihua CAI Peiquan WANG Chunxin |
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| Affiliation: | 1.Clinical Laboratory, Zhangjiagang First People＇s Hospital, Suzhou, Jiangsu Province, 215600, China;2.Wuxi People＇s Hospital Affiliated to Nanjing Medical University,Wuxi, Jiangsu Province, 214023, China) |
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| Abstract: | Objective To investigate the distribution of aminoglycoside modifying enzyme genes, 16SrRNA methylase genes and adeB efflux pump gene in extensively drug resistant acinetobacter baumannii （XDR-ABA）. Methods From January, 2011 to December, 2011, 20 strains of XDR-ABA were collected from clinical specimen. Antimicrobial susceptibility test was performed by broth microdilution method and 9 kinds of aminoglycoside modifying enzyme genes, 6 kinds of 16SrRNA methylase genes and adeB effiux pump gene were analyzed by PCR. Results All 20 strains of XDR-ABA were positive for 4 kinds of aminoglycoside modifying enzyme genes aac（6＇）- I b, ant（2＂）- I, ant（3＂）- I and aphA1 and adeB efflux pump gene, negative for other 5 kinds of aminoglycoside modifying enzyme genes and 6 kinds of 16SrRNA methylase genes in PCR assays. Conclusion In this group of XDR-ABA, 4 kinds of aminoglycoside modifying enzyme genes aac （6＂）- I b, ant （2＂）- I, ant （3＂）- I and aphA1 and adeB efflux pump system might play a role in resistance to aminoglycoside antimicrobial agents. |
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| Keywords: | Acinetobacter baumanii Aminoglycoside modifying enzyme gene 16SrRNA methylase gene Efflux pump Extensive dmg resistance |
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