鳞状细胞癌组织中HHV-8 K15等位基因型研究

目的 探讨皮肤鳞状细胞癌和食道鳞状细胞癌患者癌组织中人类疱疹病毒8型（HHV-8）K15等位基因型与肿瘤的发病关系。方法 序列特异性引物-巢式PCR技术检测40例皮肤鳞状细胞癌、40例食道鳞状细胞癌石蜡包埋组织中HHV-8 K15基因，并确定HHV-8 K15等位基因型，运用χ2检验对结果进行统计学分析。结果 9例皮肤鳞状细胞癌（22.5%）石蜡包埋组织中检测到HHV- 8 K15P型，8例食道鳞状细胞癌（20%）石蜡包埋组织中检测到 HHV- 8K15P型，两种肿瘤均未检测出K15M基因型。HHV-8在皮肤鳞状细胞癌与食道鳞状细胞癌感染率组间比较差异无统计学意义（P > 0.05）。结论 鳞状细胞癌中感染HHV-8K15P基因为K15P等位基因型，其发生发展可能与HHV-8的感染有一定的关系。

Profiling of human herpesvirus-8 (HHV-8) K15 allelotypes in squamous cell carcinoma tissue

Objective To study the relationship of HHV-8 K15 allelotypes in cutaneous and esophageal squamous cell carcinoma (SCC) tissue with tumorigenesis. Methods Sequence specific primernested PCR was performed to detect HHV-8 K15 gene and to determine its allelotype in paraffin-embedded tissue specimens from 40 patients with cutaneous SCC and 40 patients with esophageal SCC. Chi-square test was used for statistical analysis. Results HHV-8 K15 P allele was detected in 9 (22.5％) of the cutaneous SCC specimens and 8 (20％) of the esophageal SCC specimens. There was no significant difference in the detection rate of HHV-8 between cutaneous SCC and esophageal SCC specimens (P ＞ 0.05). HHV-8 K15 M allele was undetected in this study. Conclusions SCC tissues appear to harbor only HHV-8 K15 P allele, and HHV-8 may play a part in the initiation and progression of SCC.