浸润性乳腺癌HER-2基因扩增与蛋白表达的关系研究

目的 了解HER-2基因与蛋白在乳腺癌中表达状况,探讨HER-2基因与Ki-67、P53、ER、PR、年龄、淋巴结转移、TNM分期的相关性及其临床意义.方法 收集150例浸润性乳腺癌组织石蜡标本,用荧光原位杂交( FISH)技术和免疫组化(IHC)法分别检测标本中的HER-2基因及HER-2、Ki-67、P53、ER、PR蛋白表达情况.结果 150例浸润性乳腺癌标本HER-2基因扩增率为32.68％ (49/150)；其中,在HER-2蛋白(+++)的病例中HER-2基因扩增率为90.32％ (28/31)；HER-2蛋白(++)的病例中扩增率34.78％ (16/46)；HER-2蛋白(+)和(-)的扩增率分别为11.54％(3/26)、4.25％(2/47).HER-2基因与ER、PR表达及TNM分期相关,而与年龄、Ki-67、P53、淋巴结转移无关.结论 FISH技术和IHC技术检测HER-2基因和蛋白表达在(+++)和(-)的病例中一致性较好,IHC可作为替代FISH检测HER-2基因状态的方法.

A study on the relationship between HER-2 gene amplification and protein expression in invasive breast cancer

Objective To study the amplification and expression of HER-2 gene and protein in breast cancer and to investigate the relationship between HER-2 gene and Ki-67 、P53 、ER、PR、lymph node metastasis and TNM staging. Methods Fluorescence in situ hybridization (FISH) and immunohistochemistry(IHC) method were used to detect the amplification and expression of HER-2 and the expression of Ki-67、P53、ER、PR in 150 cases of breast cancer. Results 49 out of 150 cases were amplificated,positive rate of HER-2 gene was 32.68％.In the 32 patients whose expression of HER-2 protein was ( + + + ),HER-2 gene was expressed in 28 cases.In The 46 patients whose expression of HER-2 protein was ( + + ),HER-2 was expressed in 16 cases.In the 26 patients whose expression of HER-2 protein was ( + ),HER-2 was expressed in 3 cases.In the 47 cases that the expression of HER-2 protein was negative,there were 45 cases that the expression HER-2 gene was negative.The expression of HER-2 was correlated with ER,PR expression and TNM staging,but not significantly related with age,Ki-67,P53 and lymph node metastasis.Conclusions FISH and IHC correlate well with each other in the determination of HER-2 gene amplication and protein expression in cases of invasive breast cancer.