内皮抑素在肺癌患者外周血清及支气管肺泡灌洗液中的表达研究

 目的 探讨内皮抑素（Endostatin）在肺癌患者外周血清及支气管肺泡灌洗液（Bronchoalveolar lavage fluid，BALF）中的表达以及与肺癌临床病理生理特征的关系。方法 采用酶联免疫吸附法（Enzyme-linked immunosorbent assay，ELISA）检测初诊肺癌47例及肺良性病变18例患者外周血清及BALF中Endostatin的表达水平。结果 肺癌患者外周血清及BALF中Endostatin分别为（131．71±50．32）ng／ml和（502．56±302．00）ng／ml，显著高于肺良性病变者（P〈0．01）；肺癌晚期、有淋巴结及远处转移、肺腺癌患者外周血清及BALF中Endostatin高表达；肺癌患者Endostatin在外周血清及灌洗液中的表达呈线性正相关（P=0．000）。结论 检测外周血清及支气管肺泡灌洗液中Endostatin均有助于肺癌的诊断及较好提示其生物学行为。

Expression of Endostatin in Serum and in Bronchoaiveolar Lavage Fluid in Patients with Lung Cancer

Objective 　To evaluate the expression of endostatin in bronchoalveolar lavage fluid (BALF) and serum in patients with lung cancer. And to analysis the relationship between the levels of it and clinical features as well as pathophysiological characteristics. Methods 　The samples of serum and BAL F were obtained from 47 patient s with untreated primary lung cancer and 18 patients with benign pulmonary diseases. The levels of endostatin were analyzed by enzyme-linked immunosorbent assay ( ELISA) . Results 　The expression of endostatin in serum was (131. 71 ±50. 32) ng/ ml and in BALF was (502. 56 ±302. 00)ng/ ml in patient s with lung cancer. And the expression of endostatin in patient s with benign pulmonary diseases was (85. 86 ±36. 86) ng/ ml and (279. 49 ±135. 17) ng/ ml , respectively ( P < 0. 01) , there was significantly difference between them. The levels of endostatin in serum and BALF in advanced stage lung cancer patient s were higher than those of early stage patients. In adenocarcinoma patients was significantly higher than that in patients with squamous cell carcinoma and SCLC in serum and BAL F. Endostatin concent rations in BALF and in serum were significantly higher in patients with lymph node and distant metastasis than those patient s with no metastasis. The expression of endostatin in serum was closely related to that in BAL F in patients with lung cancer ( P = 0. 000) . Conclusion 　The detection of endostatin in serum and in BALF may be helpful to distinguish the lung cancer from benign pulmonary diseases and to reveal the biological behaviors of lung cancer well.