妊高征胎盘组织表达flt1蛋白的研究

目的: 研究fm 样酪氨酸激酶1(thefms liketyrosinekinase,flt1)蛋白在妊娠高血压综合征(妊高征)胎盘组织中的表达,探讨其在妊高征发病机制中的作用.方法: 应用蛋白免疫印迹技术(WesternBlotting)检测18例妊高征胎盘组织(实验组)和18例正常孕妇胎盘组织(对照组)中flt1蛋白的表达量,并比较两者之间的差异.应用免疫组化技术对上述两组标本中flt1蛋白的表达进行定位.结果: flt1蛋白在妊高征胎盘组织和正常孕妇胎盘组织中均有表达,在妊高征胎盘组织中的表达量高于正常孕妇,两者比较有显著性差异(P 0. 01).免疫组化染色结果显示flt1蛋白的表达部位在滋养细胞和血管内皮细胞的胞质、胞核,两组的表达部位无明显差异.结论:flt1蛋白在妊高征胎盘组织中高表达,可能与妊高征发病密切相关,它既表达于内皮细胞,又表达于滋养细胞,可能从多个途径参与妊高征的病理生理过程.

Evaluation of placental flt1 protein in women with pregnancy-induced hypertension syndrome

AIM: To investigate the expression of the fms-like tyrosine kinase 1 (flt1) in placentas from women with pregnancy-induced hypertension syndrome (PIH) and the significant role of flt1 in the pathogenesis of PIH.METHODS: The protein expression of flt1 was determined using Western Blotting analysis in 18 PIH placentas and 18 normotensive pregnancies and the localization of the flt1 in these human placental tissues was examined by immunohistochemistry.RESULTS: Placental flt1 protein expression was higher in women with PIH than that in the control group(P<0.01) and immunohistochemistry staining showed that the flt1 was localized in the syncytiotrophoblast and endothelia cells of villous capillaried and large vessels.CONCLUSION: The increased flt1 protein may alter VEGF-mediated function on trophoblast and endothelial cells in the placenta with PIH.