VEGF反义RNA 抑制裸鼠体内食管癌细胞生长的实验研究

 目的 探讨VEGF反义RNA对食管癌细胞在裸鼠体内生长的影响。方法 观察转染VEGF反义RNA的食管癌细胞TE-1在裸鼠体内成瘤性；应用免疫组化法、RT—PCR法检测移植瘤组织中VEGF蛋白及mRNA表达和MVD；用流式细胞仪和透射电镜检测细胞凋亡情况。结果 转染VEGF反义RNA的TE-1细胞在裸鼠体内肿瘤形成的潜伏期明显延长，所形成肿瘤的重量和体积小于对照组及空载体组。肿瘤组织中VEGF蛋白和mRNA的表达降低，MVD降低；肿瘤细胞发生明显的凋亡形态学改变；G0／G1期细胞明显增多，S期细胞明显减少，细胞增殖能力降低。结论 VEGF反义RNA能抑制裸鼠体内TE-1细胞的生长，减少肿瘤内血管的生成，增加细胞凋亡；为食管癌的基因治疗提供了一定的实验依据。

The Inhibitory Effect of VEGF Antisense RNA for Esophageal Cancer Cells in Vivo

Objective To study the inhibitory effect of antisense VEGF RNA on growth of TE- 1 cell in vivo,Methods TE-1 cells transfected with VEGF antisense RNA, pcDNA3. 1 plasrnid alone were transplanted subcutaneously into nude mice and growth of the transfected cells in vivo was investigated. The expression level of VEGF mRNA and protein.MVD were examined in antisense group by RT- PCR analysis and immunohistochemistry respectively. Apoptotic cell and cell cycle were detected in transfected cells by FCM assay and the electron micrographs. Results Latent period of the tumor formation of the antisense group was lengthened, while body weight.volume of tumors was significantly smaller than that of empty vector group and control group. The decreased expression level of VEGF protein and mRNA,MVD were found in antisene group by immunohistochemistry. Apoptotic cells were found in antisense group cells. G0/G1 phase cells were increased and S phase cells were decreased in cell cycle. Conclusions VEGF antisense RNA could inhibit the proliferation of TE-1 cell in the nude mice, which may apply a useful theory basis for gene therapy of esophageal cancer.