Measurement of hemoglobin in long-term fixed erythroid cells: application development for cell science experiments in microgravity |
| |
Authors: | Kun Xu Kerry L Davis Arthur J Sytkowski |
| |
Institution: | (1) Division of Hematology and Oncology, Laboratory for Cellular and Molecular Biology, Beth, Israel;(2) Deaconess Medical Center, Department of Medicine Boston, Harvard Medical School, Massachusetts, 02215, USA;(3) Laboratory for Cellular and Molecular Biology, Division of Hematology and Oncology, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA |
| |
Abstract: | Studying the effects of microgravity on cell differentiation will enhance our understanding of fundamental biology and is indispensable for a sustained space program. Rauscher murine erythroleukemic cells were chosen as a model system to study erythroid cell differentiation aboard the International Space Station because these cells undergo differentiation in response to the natural inducer, erythropoietin, as well as various chemical inducers. We have now developed a method to quantify hemoglobin in Rauscher cells after weeks of fixation and storage required for such space biology experiments. By exploiting the pseudoperoxidase activity of hemoglobin and by using reagents that yield a soluble chromophore that freely passes out of fixed cells, we developed a highly specific and sensitive assay applicable to cells fixed as long as 4 months. |
| |
Keywords: | Colorimetric erythropoiesis hemoglobin long-term fixation tetramethyl benzidine |
本文献已被 SpringerLink 等数据库收录! |
|