| 结核灶内抗酸菌L型检出方法的研究和意义 |
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| 引用本文： | 郭安河 张国昌. 结核灶内抗酸菌L型检出方法的研究和意义[J]. 农垦医学, 1998, 20(3): 131-133 |
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| 作者姓名： | 郭安河 张国昌 |
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| 作者单位： | [1]蚌埠铁路中心医院病理科 [2]石河子大学医学院病理教研室 |
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| 摘 要： | 本文应用Ｉｔｅｎｆｉｓｅｄ ｋｉｎｙｏｕｎ（ＩＫ）改良抗酸染色，Ｚｉｅｈｌ－Ｎｅｅｌｓｅｎ（ＺＮ）经典抗酸染色和Ｂａｃｉｌｌｕｓ Ｃａｌｍｅｔｔｅ Ｇｕｅｖｉｎ（ＢＣＧ）抗体免疫组化染色等方法对１８１例结核病组织切片进行了结核杆菌的检测。结果ＩＫ抗酸染色法Ｌ型结核菌检出率为６７．４％，ＺＮ法为２９．８％，两者差异有显著性。两种抗酸染色法均证明结核灶内主要（６７．４％、２９．８％）为抗酸菌Ｌ型；杆菌
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| 关 键 词： | IK 抗酸染色 ZN BCG 结核病 抗酸菌L型 |
Study and significance of method on detecting acid-fast bacterial L-form in tuberculosis fous |
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| Guo Anhe,et al. Study and significance of method on detecting acid-fast bacterial L-form in tuberculosis fous[J]. Agricultural Reclamation Medicine, 1998, 20(3): 131-133 |
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| Authors: | Guo Anhe et al |
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| Abstract: | 181 cases of tuberculosis were studied using Intenfised kinyoun (IK), Ziehl - Neelsen (ZN) acid - fast staining and Bacillus Calmette Guerin (BCG ) antibody immunohistochemical technique(ABC). It was showed that the detective rate of acid - fast bacterial L - form was 67. 4% (IK method ), 29. 8 % (ZN method ), which are obviously different, P<0. 005. Two acid - fast staining results indicated that acid - fast bacterial L - forms was mainly in tuberculosls focus (67. 4 %, 28. 9 % );the positive of acid-fast bacillus was only one case (both IK and ZN ). The positive rate of L - form antigen was 82. 3 %. The results indicated that the infection of acid -fast bacterial L-forms might be important causes of misdiagnosis, recurrence and spread. The IK method possibly increases detecting rate of acid - fast bacterial L - forms. |
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| Keywords: | KZ acid-fast staining ZN acid-fast staining BCG antibody immunohis-tochemical staining L-form |
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