Isolation and bioactivity of putative cholecystokinin-releasing peptide from rat small intestinal mucosa |
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Authors: | Kyoko Miyasaka MD Akihiro Funakoshi |
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Institution: | 1. Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, 173, Tokyo, Japan 2. National Kyushu Cancer Center, Fukuoka, Japan
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Abstract: | Pancreatic exocrine secretion in the conscious rat is regulated by proteases in the intestine secreted by the pancreas, and
cholecystokinin (CCK) is known to be involved in the mechanism. The authors proposed that the release of CCK was regulated
by a CCK-releasing factor secreted into the intestinal lumen from the proximal intestine. We isolated and partially purified
a CCK-releasing factor from rat small intestine by gel filtration and high performance liquid chromatography. The partially
purified CCK-releasing factor increased pancreatic exocrine secretions and plasma CCK concentrations in conscious rats and
this activity was abolished after the incubation with trypsin. The bioactivity of the partially purified CCK-releasing factor
was confirmed. |
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Keywords: | CCK release CCK-releasing peptide conscious rat pancreatic secretion |
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