Gene assignment of α-fucosidase and glucose dehydrogenase to the p21→pter region of chromosome 1 in man

Assignment of human genes coding for -fucosidase (FUC) and glucose dehydrogenase (GDH) to chromosome 1 has been confirmed and a location in the p21pter region demonstrated using man-mouse somatic cell hybrids. The regional location af FUC andGDH was established in cell hybrids using human cells possessing 1/2 translocation chromosomes [46,XX,t(1;2)(p21;q37)]. Hybrids which retained the 2q⁺ chromosome carrying the 1p211pter region concordantly expressed FUC, GDH, and the short-arm markers ENO₁, AK₂, and PGM₁. Hybrids which retained the 1p211qter region only expressed human PEPC and FH. Data obtained from hybrids in which spontaneous breaks in chromosome 1 had occurred indicate that the gene order in 1p21ar1pter is (ENO₁,GDH)-FUC-AK₂-PGM₁.