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| 高效液相色谱法测定白术挥发油中苍术酮的含量 | |
| 引用本文： | 王峰,蔡光明,郭惠玲. 高效液相色谱法测定白术挥发油中苍术酮的含量[J]. 中南药学, 2008, 6(3): 320-322 |
| 作者姓名： | 王峰 蔡光明 郭惠玲 |
| 作者单位： | 1. 中国人民解放军第302医院中医药研究所,北京,100039;江西中医学院,南昌,330006 2. 中国人民解放军第302医院中医药研究所,北京,100039 3. 江西中医学院,南昌,330006 |
| 摘 要： | 目的建立白术挥发油中主要活性成分苍术酮的含量测定方法。方法采用高效液相色谱法,Alltima HPC18色谱柱（250mm×4.6mm,5μm）,柱温为室温,流动相为甲醇-水（95∶5）,流速：1.0mL·min^-1,检测波长为220nm。结果在上述色谱条件下,苍术酮在0.24～2.04μg具有良好的线性关系（r=0.9998）,平均回收率为98.62%,RSD=1.4%。结论该方法简便、准确、快速,可用于白术挥发油及其制剂的质量控制。 |
| 关 键 词： | 苍术酮 高效液相色谱 白术 含量 |
| 文章编号： | 1672-2981(2008)03-0320-03 |
| 修稿时间： | 2008-01-15 |
Determination of Atractylon in the volatile oil of Atractylodes macrocephala Koidz by HPLC |
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| WANG Feng,CAI Guang-ming,GUO Hui-ling. Determination of Atractylon in the volatile oil of Atractylodes macrocephala Koidz by HPLC[J]. Central South Pharmacy, 2008, 6(3): 320-322 | |
| Authors: | WANG Feng CAI Guang-ming GUO Hui-ling |
| Affiliation: | WANG Feng,CAI Guang-ming,GUO Hui-ling(1.Institute of Chinese Medicine,302 Hospital of PLA,Beijing 100039;2.Jiangxi College of Traditional Chinese Medicine,Nanchang 330006) |
| Abstract: | Objective To establish an HPLC method for the determination of Atractylon in the volatile oil of Atractylodes macrocephala Koidz.Methods The HPLC analysis was carried out on Alltima HP C18 column（250 mm×4.6 mm,5 μm）at room temperature.The mobile phase was methanol-water（95∶5）.The flow rate was 1.0 mL·min^-1.The detection wavelength was 220 nm.Results The linear range of Atractylon was 0.24～2.04 μg（r=0.999 8）,the average recovery of this method was 100.59%,and the RSD was 1.28%.Conclusion The method can be used for the quality control of the volatile oil of Atractylodes macrocephala Koidz.and their preparations. |
| Keywords: | Atractylon HPLC Atractylodes macrocephala Koidz content |
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