| 多发性骨髓瘤患者骨髓间充质干细胞的生物学特性研究 |
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| 引用本文： | 赵志华,赵智刚,陆宁,王晓芳.多发性骨髓瘤患者骨髓间充质干细胞的生物学特性研究[J].实用癌症杂志,2010,25(5). |
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| 作者姓名： | 赵志华 赵智刚 陆宁 王晓芳 |
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| 作者单位： | 天津市肿瘤防治重点实验室,天津医科大学附属肿瘤医院血液肿瘤科,300020 |
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| 摘 要： | 目的研究多发性骨髓瘤(MM)患者骨髓间充质干细胞(MSCs)的生物学特性及其体外支持造血的能力。方法取15例MM患者的骨髓MSCs,并在体外培养和扩增。观察细胞形态,应用流式细胞术检测MSCs免疫表型;体外诱导MSCs向骨和脂肪分化;RT-PCR检测MSCs造血因子表达情况;将MM骨髓MSCs作为滋养层,应用甲基纤维素半固体培养,检测其支持造血能力。结果 MM来源的MSCs形态为典型的纤维细胞样,该细胞群体表达CD29、CD44、CD105,而CD14、CD31、CD34、CD45、HLA—DR均表达阴性。油红O染色和VonKossa染色检测显示MM骨髓MSCs可以向骨和脂肪分化。MM骨髓MSCs表达造血相关因子,在体外可以维持和扩增造血干/祖细胞。结论 MM患者骨髓中存在具有多向分化能力的MSCs,其具有体外支持造血的功能。
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| 关 键 词： | 多发性骨髓瘤 间充质干细胞 支持造血 |
Characterization and Functionality of Multiple Myeloma Patients Bone Marrow Mesenchymal Stem Cells |
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| ZHAO Zhi-hua,ZHAO ZHI-gang,LU-Ning,et al..Characterization and Functionality of Multiple Myeloma Patients Bone Marrow Mesenchymal Stem Cells[J].The Practical Journal of Cancer,2010,25(5). |
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| Authors: | ZHAO Zhi-hua ZHAO ZHI-gang LU-Ning |
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| Institution: | ZHAO Zhi-hua,ZHAO ZHI-gang,LU-Ning,et al.Department of Hematology,Key Labrotary of Cancer Prevention and Therapy,Tianjin Medical University Cancer Institute and Hospital,Tianjin,300020 |
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| Abstract: | Objective To study the characteristics of mesenthymal stem cells (MSCs) derived from multiple myeloma (MM) patients' bone marrow in vitro.Methods Bone marrow mononuclear cells from MM were obtained,cultured and expanded.Immunophenotype and in vitro differentiation capacities were investigated.Expression of cytokines was detected by RT-PCR,and hematopoiesis support of MSCs in vitro was detected by long term bone marrow culture and the methylcellulose progenitor assay.Results MSCs derived from MM presented a ... |
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| Keywords: | Multiple myelama Mesenchymal stem cells Hematopoiesis support |
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