改进SP法检测热休克蛋白

目的 :探索避免常规SP法检测热休克蛋白 (HSP)时出现假阳性和消除非特异性反应的方法。方法 :心肌组织石蜡切片 ,采用改进的SP法染色 ,即在常规SP法基础上“一抗”倍比稀释 ,双蒸水稀释H2 O2阻断内源性过氧化物酶等染色操作。结果 :改进的SP法染HSP阳性产物呈棕黄色细颗粒状 ,在细胞浆中弥漫分布 ,背景十分清亮。结论 :改进后的SP法更适用于HSP的检测

Examination of Heat Shock Protein (HSP) by Improved Method of SP

Establishing the improved SP methods to avoid the phe nomenon of false positine and eliminate the non specificity reaction during the test of HSP. Method: Using the wax slices of mycocardium tissue and the impro ved SP dyeing methods with "the first antibody" serial dilutions the routine t rypsim to digest the repared antigen and 3% H 2O 2 to prevent the activity of endoperoxidase.Results: These improvements can successfully avoid the phenomeno n of false positive resuct the positive products of HSP dyeing present in fine b rown particle which spread in cytoplasma and the dyeing background is very clear .Conclusion: The improved sp methods can be applied to the clinic al HSP testing.