Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry |
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| Authors: | C Qi W Zhu Y Niu H G Zhang G Y Zhu Y H Meng S Chen G Jin |
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| Institution: | Institute of Mechanics, Chinese Academy of Sciences, Beijing;;Radiation Medical Institute of Shandong Academy of Medical Sciences, Jinan;;Institute of Microcirculation, Peking Union Medical College &Chinese Academy of Medical Science, Beijing;;Deparment of Cardiology, Shandong Provincial Hospital, Jinan;;and Graduate University of Chinese Academy of Sciences, Beijing, China |
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| Abstract: | Summary. A biosensor based on imaging ellipsometry (BIE) has been developed and validated in 169 patients for detecting five markers of hepatitis B virus (HBV) infection. The methodology has been established to pave the way for clinical diagnosis, including ligand screening, determination of the sensitivity, set-up of cut-off values (CoVs) and comparison with other clinical methods. A matrix assay method was established for ligand screening. The CoVs of HBV markers were derived with the help of receiver operating characteristic curves. Enzyme-linked immunosorbent assay (ELISA) was the reference method. Ligands with high bioactivity were selected and sensitivities of 1 ng/mL and 1 IU/mL for hepatitis B surface antigen (HBsAg) and surface antibody (anti-HBs) were obtained respectively. The CoVs of HBsAg, anti-HBs, hepatitis B e antigen, hepatitis B e antibody and core antibody were as follows: 15%, 18%, 15%, 20% and 15%, respectively, which were the percentages over the values of corresponding ligand controls. BIE can simultaneously detect up to five markers within 1 h with results in acceptable agreement with ELISA, and thus shows a potential for diagnosing hepatitis B with high throughput. |
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| Keywords: | hepatitis B imaging ellipsometry label-free biosensor microfluidic |
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