大孔树脂吸附法分离纯化射干异黄酮的研究

【目的】研究大孔树脂吸附法分离纯化射干中异黄酮类成分。【方法】采用紫外分光光度计法测定射干异黄酮的含量，分别考察了HPD-300、HPD-400、NKA-9、AB-8、DM-301及D101等6种吸附树脂对异黄酮类的动态吸附及洗脱性能。【结果】HPD-300用于分离纯化异黄酮类成分，吸附和洗脱性能良好，吸附过程中上样液浓度为1．0g生药／ml树脂，pH值为7．0，流出液流速为1BV／h，树脂的饱和吸附容量为65．82mg／ml树脂；洗脱过程采用水和10％乙醇洗脱除杂，5BV70％乙醇梯度洗脱，流速为1BV／h，洗脱率在90％以上，所得固体样品中异黄酮类成分含量可达62％以上。【结论】HPD-300用于富集异黄酮类成分效果较好，是一种理想的分离纯化介质。

Studies on the separation and purification of isoflavones from belamcanda chinensis by macroporous adsorption resin

[Objective] To study the separation and purification of isoflavones from Belamcanda chinensis （L.） DC. by macroporous resins. [Methods] The dynamic adsorption and desorption characteristics of macroporous adsorption resins including HPD- 300, HPD- 400, NKA- 9, AB- 8, DM - 301 and D101 for isoflavones were investigated. At the base of the investigation, the best macroporous adsorption resin was chosen. [ Results] The adsorption and elution characteristies of HPD- 300 used to separate and purify isoflavones was very good. In the course of adsorption, the concentration of adsorption solution was 1.0 g dried medicinal herb per ml resins, with about of pH 7.0 of adsorption solution, and the flowing rate was 1BV/h In the course of elution, the resin column chromatography was eluted gradiently with 5BV 70% EtOH after removing impurities with water and 10% EtOH. The elution rates was more than 90%, and the contents of isoflavones was up to 62% in the dried fractions of 70% ethanolic elutions. [Conclusions] Macroporous adsorption resin HPD-300 is suitable for purify the total isoflavones from Belamcanda chinensis （L.） DC.