促红细胞生成素对脑出血大鼠脑血肿周围Fas相关死亡域蛋白和Caspase-8表达的影响

目的 研究大鼠脑出血不同时期Fas相关死亡域蛋白(FADD)和Caspase-8表达及促红细胞生成素(EPO)对其的影响,探讨EPO的脑保护机制.方法 SD雄性大鼠126只随机分为假手术组、脑出血组、EPO干预组各42只,各组又按不同时间点分为术后3、6、12、24、48、72 h和7 d共7个亚组(每个亚组6只).采用自体血脑内注射法建立脑出血动物模型,应用免疫组化方法 检测脑出血后不同时间点血肿周边脑组织中FADD和Caspase-8表达情况.结果 脑出血后3 h FADD和Caspase-8升高分别为(4.66±0.46)和(15.89±1.81)],48 h达到高峰分别为(35.88±4.24)和(45.04±3.99)],与脑出血组比较,EPO干预组3 h和48 h的FADD和Caspase-8表达显著减少分别为(3.92±0.64)和(28.24±1.90)、(13.32±2.01)和(35.08±2.85)].结论 脑出血后血肿周围细胞FADD和Caspase-8表达明显增多,EPO可能通过抑制FADD和Caspase-8的表达,对脑出血后脑组织有保护作用.

Experimental studies of the effect of erythropoietin on fas-associated death domain protein and caslmse-8 protein in rat with intracerebral hemorrhage

Objective To study the protein expressions of Fas-associated death domain protein (FADD) and caspase-8 in rats with intracerebral hemorrhage ,and the effects of erythropoietin tp reveal the mechanism of neu-m-protection by EPO. Methods 126 male SD rats were randomly divided into three groups: Sham-operated group, intracerebral hemorrhage group, and EPO group. Each group was divided into seven subgroups according to the differ-ent time points (3,6,12,24,48,72 h and 7 d). The model of intracerebral hemorrage was established in rats by in-tracerebral injection of autogenous blood. The protein expressions of FADD and caspas-8 in rats tissue around the hemorrhagic and the normal brain tissue were detected by immunohistochemistry. Results The protein expressions of FADD and caspase-8 were increased (4.66±0.46 ) and ( 15.89±1.81)] at 3 h after intracerebral hemorrhage, and peaked at 48 h (35.88±4.24 ) and (45.04±3.99)], the expressions of FADD and caspas-8 in the region around hematoma in EPO group significantly decreased compared with model group (3.92±0.64) and (28.24±1.90), (13.32±2.01 ) and (35.08±2.82)] at 3 h and 48 h. Conclusion The protein expressions of FADD and easpase-8 are markedly increased after intracerebral hemorrhage. EPO can protect the neurons by signifi-cantly reducing the expressions of FADD and caspase-8.