大鼠局部肌肉萎缩实验模型的设计与建立

背景:目前研究肌肉与骨骼关系的常用动物模型均存在不足.已经有研究表明A型肉毒素可以使局部肌肉萎缩,而不影响周围其他的肌肉.目的:尝试是否可以用局部肌肉注射A型肉毒素的方法建立一种较为理想的肌肉萎缩的动物实验模型.方法:4月龄雄性Wistar大鼠25只,采用氯胺酮(0.2 mL/kg)和速眠新(0.2 mL/kg)肌注麻醉,在股骨背侧中部做-0.5 cm切口,暴露股四头肌,右侧股四头肌肌注2 U(0.2 mL)注射用A型肉毒毒素,左侧股四头肌注射等量生理盐水作为对照.分别于模型建立后的1,2,4,6和8周各取5只做肌肉大体、组织学观察.结果与结论:肌肉大体观察发现:与生理盐水侧对照组相比,注射A型肉毒素侧股四头肌体积明显缩小、质量明显降低(P＜0.01);肌肉组织学观察发现:注射A型肉毒素侧股四头肌较生理盐水侧明显萎缩、肌纤维变细、肌纤维细胞核聚集、肌纤维与肌纤维之间间距变小.肉毒素侧1周和2周出现肌肉质量突降,4,6,8周之间肌肉质量增加缓慢,而各时间点生理盐水侧肌肉质量呈显著增加的趋势.通过对局部肌肉注射A型肉毒素可以理想地建立单个目的肌肉萎缩的实验模型,此模型操作性强、重复性好、特异性高,可应用于肌肉与骨骼关系的相关研究.

Design and establishment of a rat model of experimental local muscular atrophy

BACKGROUND: There were disadvantages of animal models in the study of the relationship of muscle and skeleton. Recent study has been demonstrated the effect of Botulinum toxin type A to atrophy local muscle without influencing the surrounding muscle. OBJECTIVE: To construct a reasonable animal model of local muscle atrophy by local injection of Botulinum toxin type A. METHODS: Totally 25 male Wistar rats of 4 months were subjected to ketamine (0.2 mL/kg) and Sumianxin (0.2 mL/kg) by intramuscular injection....