| PCR及限制性酶切技术检测急性髓细胞白血病H-ras基因点突变 |
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| 引用本文： | 李栋梁,宣宝和,温进坤,魏素珍.PCR及限制性酶切技术检测急性髓细胞白血病H-ras基因点突变[J].华北国防医药,1997(2). |
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| 作者姓名： | 李栋梁 宣宝和 温进坤 魏素珍 |
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| 作者单位： | 白求恩国际和平医院血液内科
(李栋梁，宣宝和)，河北医科大学基础所
(温进坤)，河北医科大学基础所(魏素珍) |
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| 摘 要： | 为了解急性髓细胞白血病(AML)H-ras基因点突变的情况,应用聚合酶链反应(PCR)和限制性酶切图谱分析技术,对55例初诊AML患者进行了检测,发现16例(29.1％)存在H-ras基因点突变,此突变与AML的发生及其某些临床指标有一定关系。H—ras基因突变可望成为AML诊断分型、指导治疗及判断预后的一项有价值的指标。
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| 关 键 词： | ras基因 突变 聚合酶链反应 髓细胞性白血病 |
Detection of H - ras Gene Point Mutation in Acute Myeloid Leukemia with PCR and Restriction Engzme Digestion Assay |
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| Li Dong- liang,Xuan Bao - he,Wun Jin - kuen,et al..Detection of H - ras Gene Point Mutation in Acute Myeloid Leukemia with PCR and Restriction Engzme Digestion Assay[J].Medical Journal of Beijing Military Region,1997(2). |
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| Authors: | Li Dong- liang Xuan Bao - he Wun Jin - kuen |
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| Institution: | Li Dong- liang,Xuan Bao - he,Wun Jin - kuen,et al. Department of Hematology Bethune International peace Hospital |
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| Abstract: | To inquire into the situation of H - ras gene point mutation in acute myeloid Leukemia (AML)55 cases with AML at diagnosis were detected. The result showed that 16 cases (29.1% ) contained H - ras gene point mutation, and the mutation had certain correlation with Leukemogenesis and some clinical parametres of AML . H - ras gene mutation will be expected to become a Valuable Predictor in diagnosis typing, directing treatment and determining outcome of AML. |
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| Keywords: | ras gene Mutation Polymerase Chain Reaction Acute myeloid Leukemia |
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