重型乙型肝炎患者血清白细胞介素-8和白细胞介素-10的检测及其临床意义

目的探讨血清白细胞介素-8(IL-8),白细胞介素-10(IL-10)在重型乙型肝炎患者中的变化及其临床意义。方法采用双单克隆抗体夹心酶联免疫吸附(ELISA)法检测32例重型乙型肝炎患者血清IL-8,IL-10水平,并以30例正常人作对照。结果重型乙型肝炎患者血清IL-8水平。显著高于正常人P<0.001。IL-10低于正常人P<0.005。血清胆红素含量愈高和凝血酶原活动度愈低,血清IL-8水平愈高P<0.05而IL-10则愈低P<0.01。重型乙型肝炎合并细菌感染患者组,重叠肝炎病毒感染组血清IL-8水平明显升高P<0.05,而IL-10则明显降低P<0.01。死亡病例比生存者血清IL-8水平显著升高P<0.01,而IL-10则明显降低P<0.01。结论检测血清IL-8水平对评估重型乙型肝炎病情严重性和预后有一定的临床参考价值。

Clinical significance of change in serum interleukin-8 and interleukin-10 levels in patients with severe viral hepatits B

Objective: To investigate the change in serum interleukin-8and-10 (IL-8, IL-10) levels and its clinical significance in severe viral hepatitis B (SH). Methods: Serum IL-8 and -10 levels were measured with sandwich enzyme linked immunosorbent assay (ELISA) in 32 patients with severe viral hepatitis B, and 30 normal controls (NC). Results: Serum IL-8 levels in.SH were obviously increased.compared to NC (P<0.001), Serum IL-10 levels in SH were obviously decreased compared to NC. The higher the serum bilirubin and the lower the prothrombin activity were, the higher would be the serum IL-8 levels and the lower would be the IL-10. Also, serum IL-8 levels in SH with bacterial infections were marked elevated compared to those without bacterial infections (P<0.05). Serum IL-10 levels in those were obviously decreased.Similarly(P<0.01). Serum IL-8 levels in nonsurvivors were significantly higher than survivors. (P<0.01) serum IL-10 levels in nonsurvivors were significantly lower than survivors (P<0.01). Conclusions: Those findings suggest that measurement of serum IL-8 and IL-10 were helpful in evaluating the clinical severity and in assessing the prognosis of the disease.