托吡酯对戊四氮诱导癫疒间大鼠体重和血清胰岛素及海马胰岛素样生长因子 1水平的影响

目的观察托吡酯(TPM)对戊四氮(PTZ)致癫疒间大鼠血清胰岛素(INS)及海马胰岛素样生长因子 1(IGF 1)水平表达的影响及二者与体重变化的关系，探讨托吡酯引起体重下降的机制。 方法佳木斯大学附属第一医院儿科于2005 03—2005 09将114只大鼠随机分为4组:空白组,TPM对照组,PTZ组,TPM治疗组，每3d测1次体重,在2、4、6周末应用放射免疫法测血清INS水平,免疫组化法测海马IGF 1表达。 结果TPM对照组、TPM治疗组在第3周末较其它两组出现体重下降，4周末降至最低点(P<005)；在4周末TPM对照组、PTZ组、TPM治疗组与空白组相比，TPM治疗组与PTZ组相比，血清INS明显降低，海马IGF 1阳性神经元细胞数明显升高(P<005)。6周末TPM治疗组与空白组、TPM对照组相比血清INS水平均显著降低(P<005)；TPM治疗组与空白组、TPM对照组、PTZ组比较，海马IGF 1阳性神经元细胞数均明显升高(P<005)。 结论托吡酯能使大鼠体重减轻,但不是通过降低大鼠血清INS水平、升高脑海马IGF 1水平实现的。另外,癫疒间大鼠血清INS降低，海马IGF 1阳性神经元细胞数水平升高，二者是否参与了癫疒间的病理生理过程尚待进一步研究。

Effects of topamax on the changes of the pentetruzole–induced epileptic rats’ weight and the levels of insulin in serum and insulin like growth factor 1 in hippocampus.

To study the effects of topamax on the serum level of insulin,the expression of insulin like growth factor 1 in hippocampus,and also their relationship with weight changes in pentetruzole induced epileptic rats.To explore the mechanism in which topamax causes the reduction of the rats weights. MethodsFrom Mar.2005 to Sep.2005 114 Wistar rats were randomly divided into four groups:the blank control group,the TPM contrast group,the PTZ model group and the TPM treated group.Weight changes were measured every 3 days.The level of serum insulin and the expression of IGF 1 in hippocampus were surveyed,using the method of radioimmunity and the immunohistochemical method respectively on the 2nd、the 4th and the 6th weekends. ResultsTPM contrast group and TPM treated group showed the reduction of the rats’ weights,comparing with the other two groups on the 3rd weekend,and then fell to the nadir on the 4th weekend(P<005).Serum insulin descended clearly but IGF 1 in hippocampus rose clearly on the 4th weekend,comparing the TPM contrast group、the PTZ group and the TPM treated group with the blank group,also the TPM treated group with the blank group(P<005).The level of IGF 1 in hippocampus rose clearly on the 6th weekend,comparing the TPM treated group with the blank group,the TPM contrast group and the PTZ group(P<005). ConclusionTopamax can reduce the weights of rats,but not by reducing the level of serum insulin and raise the level of insulin like growth factor 1 in hippocampus.Moreover,it needs further study whether the descending of the level of serum insulin and the rising of the level of insulin like growth factor 1 in hippocampus in epileptic rats both participate in the process of pathophysiology of epilepsy.