三氧化二砷对人结肠癌细胞增殖及凋亡影响的研究

目的：观察三氧化二砷（As2O3）对人结肠腺癌LS-174T细胞增殖的抑制及凋亡的诱导作用。方法：应用MTT法、细胞集落形成试验、流式细胞仪技术和TUNEL法观察不同浓度的As2O3(1.0μmol,2.0μmol，4.0μmol，8.0μmol，16.0μmol/L）对LS-174T细胞增殖和凋亡的影响。免疫组化技术观察As2O3对bcl-2基因表达的影响。结果：LS-174T细胞经As2o3作用后，细胞生长抑制率上升，细胞集落形成率下降，细胞周期中G1期细胞比例上升，S期和G2/M期细胞比例下降，TUNEL法显示凋亡细胞数增加，免疫组化结果显示bcl-2的表达明显减弱。结论：As2O3能抑LS-174T细胞增殖并诱导该细胞株凋亡，其机理可能与下调bcl-2表达有关。

Effects of arsenic trioxide(As2O3)on the proliferation and apoptosis of hunan colorectal cancer cell line LS-174T

Objective:To explore the effects of As 2O 3 on the propliferation and apoptosis of human colorectal cancer cell line LS-174T. Methods: The inhibitive effect of As 2O 3 on LS-174T was detected by MTT, and colony-forming rate of LS-174T was measured by colony forming assay. Flow cytometry was employed to assess the cell cycle of LS-174T and RUNEL was used to determine the apoptosis index of LS-1741. The expression of bcl-2 gene was observed by immunohistochemical analysis. Results:The inhibition index of LS-174T increased while the colony-forming rate decreased, with elevated apoptosis rate. The proportion of G 1 phase cells increased while that of G 2/M and S phase cells decreased in the cell cycle. The immunohistochemical analysis rrevealed significantly reduced expression of bcl-2 gene. Conclusion:As 2O 3 can inhibit the proliferation and induce the apoptosis of cell line LS-174T, possibly due to the decrease of bcl-2 gene expression.