端粒酶活性在非小细胞肺癌不同组织细胞中表达的研究

目的 对比研究端粒酶活性在非小细胞肺癌癌组织、癌旁组织、血液及痰液中的表达 ,以了解其临床意义。 方法 应用聚合酶联反应 -端粒重复序列扩增法 (PCR -TRAP)检测30例肺癌手术患者癌组织、癌旁组织、血液及痰液中的端粒酶活性 ,并以25例肺部良性疾病手术患者为对照组。结果 肺癌患者肺癌组织端粒酶活性阳性率83.3% (25/30) ,癌旁组织端粒酶活性阴性 ,外周血中端粒酶活性阳性率33.3 % (10/30) ,痰液标本中端粒酶活性阳性率56.7% (17/30)。对照组正常肺组织标本和外周血中端粒酶活性均阴性 ,痰液标本中端粒酶活性阳性率24.0% (6/25) ;两组病变组织、血液和痰液中端粒酶活性阳性率的差别均有显著性意义 (均P<0.05)。 结论 (1)非小细胞肺癌患者在肺癌组织、血液及痰液中均有端粒酶活性表达 ,其活性表达强度依次为肺癌组织、痰液、血液。 (2)痰液中端粒酶活性检测对肺部良恶性疾病的鉴别具有一定的诊断意义。

The expression of telomerase activity in patients with non-small cell lung cancer

Objective To investigate the expression of the telomerase activity in patients with non-small cell lung cancer.Methods The activity of telomerase in primary foci,paratumor tissue,blood and sputum of30patients with non-small cell lung cancer were measured with PCR-TRAP;the results were compared with those of25cases of benign lung diseases.Results In the control group,the telomerase activity in normal lung tissue and peripheral blood was negative,the positive rate of activity was24.0%(6/25)in sputum.In the cancer group,the positive rate in primary foci,peripheral blood and sputum were83.3%(25/30),33.3%(10/30)and56.7%(17/30)respectively,but negative in paratumor tissue.The positive rate between two groups was signif-icantly different(P<0.05).Conclusions(1)In patients with non-small cell lung cancer,the activity of telomerase expressed in primary foci,sputum and peripheral blood.(2)Detecting the activity of telomerase in sputum may be used in differential diagnosis between benign and malignant lung diseases.