In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits |
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Authors: | Deliorman Orhan Didem Hartevioğlu Ali Küpeli Esra Yesilada Erdem |
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Affiliation: | Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 6330, Ankara, Turkey. didem@gazi.edu.tr |
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Abstract: | The aqueous and ethanol extracts of Rosa canina L. (Rosaceae) fruits and the fractions prepared from the latter were investigated for their anti-inflammatory and antinociceptive activities in several in vivo experimental models. The ethanolic extract was shown to possess significant inhibitory activity against inflammatory models (i.e., carrageenan-induced and PGE(1)-induced hind paw edema models, as well as on acetic acid-induced increase in a capillary permeability model) and on a pain model based on the inhibition of p-benzoquinone-induced writhing in mice. Hexane, chloroform, ethylacetate, n-butanol and the remaining water fractions were obtained through bioassay-guided fractionation. Ethylacetate and n-butanol fractions displayed potent anti-inflammatory and antinociceptive activities at a dose of 919 mg/kg without inducing acute toxicity. Further attempts to isolate and define the active constituent(s) were inconclusive, possibly due to the synergistic interaction of components in the extract. |
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Keywords: | AcOH, acetic acid ASA, acetyl salicylic acid CMC, carboxymethyl cellulose LT, leukotrienes TLC, thin layer chromatography TPA, 12-O-tetradecanoylphorbol 13-acetate PGE1, prostaglandin E1 |
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